Prenylated proteins: the structure of the isoprenoid group.

Prenylated proteins: the structure of the isoprenoid group.
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DOI：
10.1126/science.2296720
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发表时间：
1990-01
期刊：
Science
影响因子：
56.9
通讯作者：
H. Rilling;E. Breunger;W. W. Epstein-W.;P. F. Crain
H. Rilling;E. Breunger;W. W. Epstein-W.;P. F. Crain
中科院分区：
综合性期刊1区
文献类型：
--
作者：
H. Rilling;E. Breunger;W. W. Epstein-W.;P. F. Crain

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中国仓鼠卵巢细胞异戊二烯化蛋白的甲羟戊酸衍生部分已被确定为二萜类化合物（C20）。该基团与羧基末端半胱氨酸连接为硫醚。通过肼解，然后通过阮内镍脱硫将其从蛋白质中除去，并通过气相色谱-质谱法分析所得的烃馏分。
The mevalonate-derived portion of a prenylated protein from Chinese hamster ovary cells has been established as diterpenoid (C20). This group is linked to a carboxyl-terminal cysteine as a thioether. It was removed from the protein by hydrazinolysis followed by Raney nickel desulfurization, and the resulting hydrocarbon fraction was analyzed by gas chromatography-mass spectrometry.