Two-photon live imaging of single corneal stem cells reveals compartmentalized organization of the limbal niche.
Two-photon live imaging of single corneal stem cells reveals compartmentalized organization of the limbal niche.
复制标题
DOI：
10.1016/j.stem.2021.02.022
复制
发表时间：
2021-07-01
期刊：
影响因子：
23.9
通讯作者：
Rompolas P
中科院分区：
文献类型：
--
作者：
Farrelly O;Suzuki-Horiuchi Y;Brewster M;Kuri P;Huang S;Rice G;Bae H;Xu J;Dentchev T;Lee V;Rompolas P
支持成体器官的常驻干细胞的功能异质性尚未完全了解。在这里，我们直接可视化的角膜利姆布斯在活体小鼠的眼睛，并确定离散的干细胞龛隔室。通过记录单个干细胞及其后代的生命周期，我们直接分析了它们的命运，并表明它们在组织中的位置可以预测它们的分化状态。内利姆布斯中的干细胞经历大部分对称分裂，并且需要维持支持角膜稳态的瞬时祖细胞群体。使用原位光标记，我们捕捉到它们的后代在一致向心移动之前离开生态位。长期牵连的慢循环干细胞在功能上是不同的，并在稳态期间显示局部克隆动力学，但可以有助于损伤后的角膜再生。这项研究证明了功能多样的干细胞群体的区室化组织如何支持成体器官的维持和再生。Farrelly等人捕获了活体小鼠眼表上皮在稳态和再生期间的动态细胞行为。这项研究表明，利姆布斯窝藏功能离散的干细胞群体，显示不同的克隆活动。
The functional heterogeneity of resident stem cells that support adult organs is incompletely understood. Here, we directly visualize the corneal limbus in the eyes of live mice and identify discrete stem cell niche compartments. By recording the lifecycle of individual stem cells and their progeny, we directly analyze their fates and show that their location within the tissue can predict their differentiation status. Stem cells in the inner limbus undergo mostly symmetric divisions and are required to sustain the population of transient progenitors that support corneal homeostasis. Using in situ photo-labeling, we captured their progeny exiting the niche before moving centripetally in unison. The long-implicated slow-cycling stem cells are functionally distinct and display local clonal dynamics during homeostasis, but can contribute to corneal regeneration after injury. This study demonstrates how the compartmentalized organization of functionally diverse stem cell populations support the maintenance and regeneration of an adult organ. Farrelly et al. capture dynamic cell behaviors across the ocular surface epithelium of live mice during homeostasis and regeneration. This study shows that the limbus harbors functionally discrete stem cell populations that display distinct clonal activities.
登录
查看更多内容
DOI：
10.3791/53370
发表时间：
2015-12-18
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Amitai-Lange A;Berkowitz E;Altshuler A;Dbayat N;Nasser W;Suss-Toby E;Tiosano B;Shalom-Feuerstein R
通讯作者：
Shalom-Feuerstein R
DOI：
10.1523/jneurosci.6451-10.2011
发表时间：
2011-03-30
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Cavanaugh DJ;Chesler AT;Jackson AC;Sigal YM;Yamanaka H;Grant R;O'Donnell D;Nicoll RA;Shah NM;Julius D;Basbaum AI
通讯作者：
Basbaum AI
影响因子：
1.2
作者：
Dorà NJ;Hill RE;Collinson JM;West JD
通讯作者：
West JD
影响因子：
64.8
作者：
Busch, Katrin;Klapproth, Kay;Rodewald, Hans-Reimer
通讯作者：
Rodewald, Hans-Reimer
影响因子：
5.2
作者：
Di Girolamo, N.;Bobba, S.;Lyons, J. G.
通讯作者：
Lyons, J. G.