Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine
Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine
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DOI：
10.1007/s00335-013-9497-4
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发表时间：
2014-01
期刊：
影响因子：
2.5
通讯作者：
G. Reiner;Natalie Bertsch;D. Hoeltig;Martin Selke;H. Willems;G. Gerlach;B. Tuemmler;I. Probst;R. Herwig;M. Drungowski;K. Waldmann
中科院分区：
文献类型：
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作者：
G. Reiner;Natalie Bertsch;D. Hoeltig;Martin Selke;H. Willems;G. Gerlach;B. Tuemmler;I. Probst;R. Herwig;M. Drungowski;K. Waldmann
胸膜肺炎放线杆菌是全世界养猪生产中最重要的病原体之一。该药剂可因性能下降、急性或慢性胸膜肺炎以及死亡发生率增加而造成严重的经济损失。治疗方法无法以可持续的方式使用，疫苗接种也并不总是可行，但更多地了解宿主防御和疾病机制可能会带来新的预防方法。本研究的目的是检测与 A 抗性/易感性相关的数量性状基因座 (QTL)。胸膜肺炎。在受控条件下，汉普夏/长白家族的 170 只 F2 动物，在创建群体中关于 A 的已知差异。胸膜肺炎耐药性，用 anA 进行攻击。胸膜肺炎 7 型气雾剂，然后进行详细的临床、放射学、超声检查、病理学和细菌学检查。使用 159 个微卫星标记对 F2 猪进行基因分型。在野猪染色体 (SSC) 2、6、12、13、16、17 和 18 上鉴定出显着的 QTL。它们解释了 6-22% 的表型变异。 SSC2 上的一个 QTL 在全基因组水平上对五种相关表型性状达到显着性。多元回归分析揭示了标记物SWR345（SSC2）和S0143（SSC12）对呼吸健康评分、临床评分和死亡发生的组合效应。结果表明A的遗传背景。猪胸膜肺炎耐药性，并为猪胸膜肺炎耐药性/易感性的遗传结构提供新的见解。结果将有助于确定潜在的基因和机制。
Actinobacilluspleuropneumoniaeis among the most important pathogens worldwide in pig production. The agent can cause severe economic losses due to decreased performance, acute or chronic pleuropneumonia and an increased incidence of death. Therapeutics cannot be used in a sustainable manner, and vaccination is not always available, but discovering more about host defence and disease mechanisms might lead to new methods of prophylaxis. The aim of the present study was to detect quantitative trait loci (QTL) associated with resistance/susceptibility toA. pleuropneumoniae. Under controlled conditions, 170 F2 animals of a Hampshire/Landrace family, with known differences in founder populations regardingA. pleuropneumoniaeresistance, were challenged with anA. pleuropneumoniaeserotype 7 aerosol followed by a detailed clinical, radiographic, ultrasonographic, pathological and bacteriological examination. F2 pigs were genotyped with 159 microsatellite markers. Significant QTL were identified onSus scrofachromosomes (SSC) 2, 6, 12, 13, 16, 17 and 18. They explained 6–22 % of phenotypic variance. One QTL on SSC2 reached significance on a genome-wide level for five associated phenotypic traits. A multiple regression analysis revealed a combinatory effect of markersSWR345(SSC2) andS0143(SSC12) on Respiratory Health Score, Clinical Score and the occurrence of death. The results indicate the genetic background ofA. pleuropneumoniaeresistance in swine and provide new insights into the genetic architecture of resistance/susceptibility to porcine pleuropneumonia. The results will be helpful in identifying the underlying genes and mechanisms.