ACTION OF GUANIDINE ON REPLICATION OF POLIOVIRUS RNA
ACTION OF GUANIDINE ON REPLICATION OF POLIOVIRUS RNA
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DOI：
10.1016/0042-6822(68)90219-5
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发表时间：
1968-01-01
期刊：
影响因子：
3.7
通讯作者：
TAMM, I
中科院分区：
文献类型：
--
作者：
CALIGUIRI, LA;TAMM, I
胍 (1.5mM) 抑制活跃产生病毒的细胞中的病毒 RNA 合成。研究发现，在病毒生长周期的第四小时后，病毒RNA合成的速率逐渐降低，胍对病毒RNA合成的抑制作用逐渐减弱。3小时添加胍对单链RNA合成的抑制速度快于对双链RNA合成的抑制速度；然而，35S单链RNA仍然是形成的主要RNA部分。虽然复制中间体中抗核糖核酸酶RNA的累积放射性净减少，但添加胍后双链复制形式的放射性量增加。这些结果表明，胍不会抑制病毒RNA链的生长和完成，但会阻止新链的起始。
Guanidine (1.5mM) inhibits viral RNA synthesis in cells that are actively producing virus. It was found that synthesis of viral RNA becomes progressively less inhibitable by guanidine after the fourth hour in the viral growth cycle when the rate of viral RNA synthesis diminishes.Guanidine added at 3 hours inhibited the synthesis of single-stranded RNA faster than synthesis of double-stranded RNA; however, 35 S single-stranded RNA was still the major RNA moiety formed. While there was a net reduction in the cumulative radioactivity of ribonuclease-resistant RNA in the replicative intermediate, there was an increase in the amount of radioactivity in the double-stranded replicative form after guanidine was added.These results indicate that guanidine does not inhibit growth and completion of viral RNA chains, but blocks the initiation of new chains.