Intrachromosomal recombination in Saccharomyces cerevisiae: reciprocal exchange in an inverted repeat and associated gene conversion.
Intrachromosomal recombination in Saccharomyces cerevisiae: reciprocal exchange in an inverted repeat and associated gene conversion.
复制标题
酿酒酵母中的染色体内重组：反向重复中的相互交换和相关基因转换。
DOI：
10.1093/genetics/117.4.633
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发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
Klein,HL
中科院分区：
文献类型：
--
作者：
Willis,KK;Klein,HL
染色体内的基因转换没有显示出很强的协会与互惠交流。然而，相互交换确实发生在染色体内重复序列之间。为了了解重复序列中相互交换与基因转换之间的关系，研究了反向重复序列的重组行为。我们已经发现，在一个方向上，细菌转座子Tn903的Kanr基因的一个单拷贝侧翼的部分反向重复序列IS903不会在酿酒酵母中产生G418抗性。IS903重复序列中的相互交换使Kanr基因反转，从而在单个拷贝中产生G4 18抗性。使用此作为染色体内相互交换的选择，我们已经引入了多个限制性位点的异源性的IS903重复序列，并检查相关的基因转换的交叉产品。在有丝分裂和减数分裂中，大约50%的交换与基因转换有关。这表明，这些交换是由一个中间体产生的，该中间体在50%的事件中产生基因转换，也就是说，重复序列之间的相互交换和基因转换具有共同的起源。这些数据与异源双链体错配修复机制最为一致。
Intrachromosomal gene conversion has not shown a strong association with reciprocal exchanges. However, reciprocal exchanges do occur between intrachromosomal repeats. To understand the relationship between reciprocal exchange and gene conversion in repeated sequences the recombi- nation behavior of an inverted repeat was studied. We have found that in one orientation a single copy of theKanrgene of the bacterial transposon Tn903 flanked by part of the inverted repeats IS903 does not give G418 resistance inSaccharomyces cerevisiae. A reciprocal exchange in the IS903 repeats inverts theKanrgene, which then gives G4 18 resistance in a single copy. Using this as a selection for intrachromosomal reciprocal exchange we have introduced multiple restriction site heterologies into the IS903 repeats and examined the crossover products for associated gene conversions. Approximately 50% of crossovers, both in mitosis and meiosis, were associated with a gene conversion. This suggests that these crossovers result from an intermediate that gives a gene conversion in 50% of the events, that is, both reciprocal exchange and gene conversion between repeated sequences have a common origin. The data are most consistent with a heteroduplex mismatch repair mechanism.