Involvement of reactive oxygen species (ROS) in the induction of genetic instability by radiation
Involvement of reactive oxygen species (ROS) in the induction of genetic instability by radiation
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DOI：
10.1269/jrr.45.181
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发表时间：
2004-06-01
影响因子：
2
通讯作者：
Watanabe, M
中科院分区：
文献类型：
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作者：
Tominaga, H;Kodama, S;Watanabe, M
辐射产生与细胞分子（包括DNA、脂质和蛋白质）相互作用的活性氧（ROS）。要知道如何ROS有助于诱导遗传不稳定性，我们研究了抗ROS条件的影响，使用抗坏血酸磷酸盐（APM）处理或低氧条件下，诱导延迟生殖细胞死亡和延迟染色体畸变。小鼠m5 S衍生的cl.将用6戈伊X射线照射的2011-14细胞重新铺板并使其形成次级集落。抗ROS处理应用于照射前培养或照射后培养，用于初级或次级集落形成。这两种抗ROS条件缓解X射线诱导的急性细胞杀伤到类似的程度。这些抗ROS条件也缓解了遗传不稳定性时，这些条件被施加在初级集落形成。然而，没有观察到的影响时，在预照射培养和二次集落形成的条件。我们还证明，在X射线照射细胞的ROS的量迅速增加，然后在6 hr postradiation下降，和ROS的水平，然后逐渐下降到基线内2周。APM处理使ROS产生保持在低于未处理对照的水平。这些结果表明，遗传不稳定的原因可能是固定的ROS在2周的照射后期间。
Radiation generates reactive oxygen species (ROS) that interact with cellular molecules, including DNA, lipids, and proteins. To know how ROS contribute to the induction of genetic instability, we examined the effect of the anti-ROS condition, using both ascorbic acid phosphate (APM) treatment or a low oxygen condition, on the induction of delayed reproductive cell death and delayed chromosome aberrations. The primary surviving colonies of mouse m5S-derived cl. 2011-14 cells irradiated with 6 Gy of X-rays were replated and allowed to form secondary colonies. The anti-ROS treatments were applied to either preirradiation culture or postirradiation cultures for primary or secondary colony formation. Both anti-ROS conditions relieved X-ray-induced acute cell killing to a similar extent. These anti-ROS conditions also relieved genetic instability when those conditions were applied during primary colony formation. However, no effect was observed when the conditions were applied during preirradiation culture and secondary colony formation. We also demonstrated that the amounts of ROS in X-ray-irradiated cells rapidly increase and then decrease at 6 hr postirradiation, and the levels of ROS then gradually decrease to a baseline within 2 weeks. The APM treatment kept the ROS production at a lower level than an untreated control. These results suggest that the cause of genetic instability might be fixed by ROS during a 2-week postirradiation period.