Detection of apoptosis by TUNEL assay.
Detection of apoptosis by TUNEL assay.
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DOI：
10.1007/978-1-61779-860-3_5
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发表时间：
2012-01-01
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影响因子：
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通讯作者：
Kioussi, Chrissa
中科院分区：
文献类型：
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作者：
Kyrylkova, Kateryna;Kyryachenko, Sergiy;Kioussi, Chrissa
末端脱氧核苷酸转移酶(TdT)dUTP缺口末端标记法(TUNEL)被设计用来检测在细胞凋亡后期经历广泛DNA降解的凋亡细胞。该方法是基于TDT独立于模板标记双链DNA断裂的钝端的能力。本章描述了一种使用TUNEL比色系统检测小鼠牙齿发育过程中的凋亡细胞的方法。
Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.