Cycles of vascular plexus formation within the nephrogenic zone of the developing mouse kidney
Cycles of vascular plexus formation within the nephrogenic zone of the developing mouse kidney
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DOI：
10.1038/s41598-017-03808-4
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发表时间：
2017-06-12
影响因子：
4.6
通讯作者：
Davies, Jamie A.
中科院分区：
文献类型：
--
作者：
Munro, David A. D.;Hohenstein, Peter;Davies, Jamie A.
肾血管系统是血液过滤、血压调节、pH值维持以及其他特殊肾脏功能所必需的。然而，尽管它很重要，但人们对其发展的许多方面知之甚少。为了提供肾脏血管化的详细时空分析，我们收集了胚胎小鼠肾脏在不同发育时间点的图像。在这里，我们描述了肾脏血管化的第一阶段，并证明了多边形血管网络（内皮丛）在肾脏周围循环形成。我们发现肾脏血管化开始于E11，此时连接到胚胎循环的血管在输尿管芽周围形成一个环。从E13.5开始，内皮丛在帽间充质细胞和输尿管芽细胞群周围以周期性、可预测的方式组织。具体来说，当输尿管芽分叉时，随着帽间质分裂，内皮细胞在分叉部位形成。神经丛是血管状的，携带红细胞，被包围在基底膜内，总能追溯到肾动脉。我们的结果是朝着理解肾脏血管系统的整体结构是如何实现的重要一步。
The renal vasculature is required for blood filtration, blood pressure regulation, and pH maintenance, as well as other specialised kidney functions. Yet, despite its importance, many aspects of its development are poorly understood. To provide a detailed spatiotemporal analysis of kidney vascularisation, we collected images of embryonic mouse kidneys at various developmental time-points. Here we describe the first stages of kidney vascularisation and demonstrate that polygonal networks of vessels (endothelial plexuses) form in cycles at the periphery of the kidney. We show that kidney vascularisation initiates at E11, when vessels connected to the embryonic circulation form a ring around the ureteric bud. From E13.5, endothelial plexuses organise around populations of cap mesenchymal and ureteric bud cells in a cyclical, predictable manner. Specifically, as the ureteric bud bifurcates, endothelia form across the bifurcation site as the cap mesenchyme splits. The plexuses are vascular, carry erythrocytes, are enclosed within a basement membrane, and can always be traced back to the renal artery. Our results are a major step towards understanding how the global architecture of the renal vasculature is achieved.