Non-ionic surfactant vesicles mediated transcutaneous immunization against hepatitis B
Non-ionic surfactant vesicles mediated transcutaneous immunization against hepatitis B
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DOI：
10.1016/j.intimp.2011.05.007
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发表时间：
2011-10-01
影响因子：
5.6
通讯作者：
Dixit, V. K.
中科院分区：
文献类型：
--
作者：
Maheshwari, Chetan;Pandey, R. S.;Dixit, V. K.
经皮免疫接种（TI）与胃肠外给药途径相比具有许多实际优点。在本研究中，非离子表面活性剂泡囊载体，即囊泡，用于局部递送疫苗，使用乙型肝炎B表面蛋白作为抗原和霍乱毒素B作为佐剂。对囊泡的大小、形状、包封率和过程中抗原稳定性进行了表征。使用人尸体皮肤进行抗原的体外渗透和皮肤沉积研究。通过共聚焦激光扫描显微镜评估囊泡的皮肤渗透效率。通过测量Balb/c小鼠经皮免疫后的血清IgG滴度、同种型比IgG 2a/IgG 1和粘膜免疫应答来研究这些囊泡的免疫刺激活性，并将结果与肌内给予的明矾吸附的HBsAg和局部给予的普通HBsAg溶液进行比较。结果显示，最佳的囊泡体制剂可以包封58.11 ± 0.71 μ m的抗原，囊泡尺寸范围为2.83 ± 0.29 μ m。连续3次局部给药后的血清IgG滴度明显优于单次给药肝炎抗原与囊泡系统，表明有效刺激血清免疫应答;较高的IgG 1/IgG 2a比值表明CTB混合囊泡引起Th 1和Th 2应答。这项研究表明，局部免疫与霍乱毒素B是潜在的佐剂皮肤免疫反应时，共同管理的HBsAg封装的囊泡。结果还表明，所研究的囊泡系统可以有效地作为疫苗的局部递送。(C)2011 Elsevier B. V.保留所有权利。
Transcutaneous immunization (TI) has many practical merits compared to parenteral routes of administration. In the present study, non ionic surfactant vesicular carrier, i.e. niosomes, was evaluated for topical delivery of vaccines using hepatitis B surface protein as an antigen and cholera toxin B as an adjuvant. Niosomes were characterized for size, shape, entrapment efficiency and in process antigen stability. In vitro permeation and skin deposition studies of antigen were performed using human cadaver skin. Skin penetration efficiency of niosomes was assessed by confocal laser scanning microscopy. The immune stimulating activity of these vesicles was studied by measuring the serum IgG titer, isotype ratio IgG2a/IgG1 and mucosal immune responses following transcutaneous immunization in Balb/c mice and results were compared with the alum adsorbed HBsAg given intramuscularly and topically administered plain HBsAg solution. The result shows that optimal niosomal formulation could entrap 58.11 +/- 0.71 of antigen with vesicle size range of 2.83 +/- 0.29 mu m. Serum IgG titers after three consecutive topical administrations were significantly better than single administration of hepatitis antigen with niosomal system, suggesting an effective stimulation of serum immune response; higher IgG1/IgG2a ratio revealed CTB mixed niosomes elicit both Th1 and Th2 responses. This study suggests that topical immunization with cholera toxin B is potential adjuvant for cutaneous immune responses when coadministered with the HBsAg encapsulated niosomes. Results also suggest that the investigated niosomes systems can be effective as topical delivery of vaccines. (C) 2011 Elsevier B.V. All rights reserved.