Two G-box like elements essential to high gene expression of SlAKR4B in tomato leaves.
Two G-box like elements essential to high gene expression of SlAKR4B in tomato leaves.
复制标题
番茄叶片中 SlAKR4B 基因高表达所必需的两个 G 盒样元件。
DOI：
10.1080/09168451.2018.1429887
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Fujikawa Y and Esaka M
中科院分区：
文献类型：
--
作者：
Suekawa M;Fujikawa Y and Esaka M
醛酮还原酶（Aldo-keto reductases，AKR）在植物醛类解毒、初级代谢和次级代谢中起重要作用。我们先前报道了水杨酸和茉莉酸处理的番茄叶片中诱导表达a Solanum lycopersicumAKR 4 B（SlAKR 4 B），并且SlAKR 4 B在番茄叶片原生质体中具有高启动子活性。本研究以番茄叶片为材料，研究了渗透处理对SlAKR 4基因表达的影响，以及SlAKR 4高启动子活性的调控元件。番茄叶片原生质体和缓冲液渗透番茄叶片中的基因表达分析表明，细胞损伤导致SlAKR 4 B的表达增加。两个G-box样元件的突变显著降低了SlAKR 4 B的启动子活性。这表明两个G-box样元件是负责高启动子活性。
Aldo-keto reductases (AKRs) play important roles in aldehyde detoxification as well as primary and secondary metabolism in plants. We previously reported inducible expression of aSolanum lycopersicumAKR4B (SlAKR4B) in tomato leaves treated with salicylic acid and jasmonic acid, and high promoter activity ofSlAKR4Bin tomato leaf protoplasts. In this study, we investigated the expression response ofSlAKR4Bin the tomato leaves with infiltration treatment and thecis-element(s) involved in high promoter activity. Gene expression analysis in tomato leaf protoplasts and buffer-infiltrated tomato leaves suggested that cell damage caused the increased expression ofSlAKR4B. Promoter activity ofSlAKR4Bwas significantly reduced by mutation of two G–box like elements. It is suggested that the two G–box like elements are responsible for the high promoter activity.