Transfer and expression of recombinant nitric oxide synthase genes in the cardiovascular system.
Transfer and expression of recombinant nitric oxide synthase genes in the cardiovascular system.
复制标题
重组一氧化氮合酶基因在心血管系统中的转移和表达。
DOI：
10.1016/s0165-6147(98)01190-0
复制
发表时间：
1998
影响因子：
13.8
通讯作者：
Katusic,ZS
中科院分区：
文献类型：
--
作者：
Chen,AF;O'Brien,T;Katusic,ZS
基因治疗涉及将功能基因转移到宿主细胞中，以纠正特定基因的功能障碍或减轻疾病的症状。对于将基因转移到心血管系统，腺病毒载体是最有效的转移手段。最近，重组一氧化氮合酶（NOS）基因向脑和心血管床的转移和功能表达已在离体和体内得到证实。在此，Alex Chen及其同事回顾了血管NOS基因转移领域的最新进展以及NOS基因治疗许多心血管疾病的潜在用途。尽管NOS基因转移方法的可行性已在动物模型中得到证实，但目前可用的载体存在许多技术和安全限制，在尝试人类NOS基因治疗心血管疾病之前必须解决这些限制。
Gene therapy involves the transfer of a functional gene into host cells to correct the malfunction of a specific gene or to alleviate the symptoms of a disease. For gene transfer to the cardiovascular system, adenoviral vectors are the most efficient means of transfer. Recently, transfer and functional expression of recombinant nitric oxide synthase (NOS) genes to cerebral and cardiovascular beds have been demonstrated bothex vivoandin vivo.Here, Alex Chen and colleagues review current progress in the field of vascular NOS gene transfer and the potential use of NOS gene therapy for a number of cardiovascular diseases. Although the feasibility of the NOS gene transfer approach has been demonstrated in animal models, currently available vectors have a number of technical and safety limitations that have to be solved before human NOS gene therapy for cardiovascular disease can be attempted.