Neurosphere-derived neural cells show region-specific behaviour in vitro
Neurosphere-derived neural cells show region-specific behaviour in vitro
复制标题
DOI：
10.1097/wnr.0b013e3282f03d54
复制
发表时间：
2007-10-08
期刊：
影响因子：
1.7
通讯作者：
Simonneau, Lionel
中科院分区：
文献类型：
--
作者：
Armando, Sylvain;Lebrun, Aurore;Simonneau, Lionel
神经球培养物为研究神经干细胞/祖细胞（NSC/NPC）提供了有用的模型。然而，神经球（NS）在体外保持其区域身份的程度受到质疑。在此，在分化后比较从小鼠胚胎皮层、纹状体或脊髓获得的NS。皮质INS的神经元形成有序的星形胶质细胞簇，纹状体NS的神经元在星形胶质细胞层的边缘形成外环，而脊髓NS的神经元像星形胶质细胞一样放射状分布。这种在体外的神经行为是区域特异性的，并坚持在克隆条件下，提供证据的位置线索在NS文化的维护。
Neurosphere cultures provide a useful model to study neural stem/progenitor cells (NSC/NPCs). The degree to which neurospheres (NS) retain their regional identity in vitro has, however, been questioned. Here, NS obtained from mouse embryonic cortex, striatum or spinal cord were compared after differentiation. Neurons from cortical INS formed well ordered clusters containing astrocytes, those from striatal NS formed an external ring at the border of the astrocyte layer, whereas those from spinal cord NS spread radially like the astrocytes. Such in-vitro neural behaviour was region-specific and persisted in clonal conditions, providing evidence of the maintenance of positional cues in NS cultures.