ONTOGENY OF IGM AND IGM-BEARING CELLS IN RAINBOW-TROUT
ONTOGENY OF IGM AND IGM-BEARING CELLS IN RAINBOW-TROUT
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DOI：
10.1016/0145-305x(93)90033-m
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发表时间：
1993-09-01
影响因子：
2.9
通讯作者：
VILLENA, AJ
中科院分区：
文献类型：
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作者：
CASTILLO, A;SANCHEZ, C;VILLENA, AJ
我们研究了虹鳟鱼 Oncorhynchus mykiss 中免疫球蛋白 M (IgM) 和携带 IgM 的细胞的个体发育。在孵化前 12 天（14 摄氏度）的胚胎中首次观察到显示细胞质 IgM 的淋巴细胞。在此阶段，不存在表面 IgM 呈阳性的细胞。在孵化前 8 天（14 摄氏度）观察到携带表面 IgM 的淋巴细胞。未受精的鳟鱼卵含有可检测量的 IgM（11.2 +/- 2.6 杯/克卵重），表明鲑鱼中可能发生 IgM 从母体到胚胎的转移。在表达表面 IgM 的胚胎内细胞出现后，整条鱼的 IgM 水平缓慢增加。组织的 IgM/g 量在孵化前后达到峰值，但该参数在孵化后 2 个月内显示较低值。
We have studied the ontogenic development of immunoglobulin M (IgM) and of IgM-bearing cells in the rainbow trout, Oncorhynchus mykiss. Lymphocytes showing cytoplasmic IgM were first observed in embryos at 12 days before hatch (14-degrees-C). At this stage, no cells positive for surface IgM were present. Lymphocytes bearing surface IgM were observed at 8 days before hatch (14-degrees-C). Unfertilized trout eggs contained detectable amounts of IgM (11.2 +/- 2.6 mug/g of egg weight), indicating that transfer of IgM from mother to embryo can occur in salmonids. The levels of IgM from whole fish increase slowly after the appearance of intraembryonic cells that express surface IgM. The amount of IgM/g of tissue peaks around hatch, but this parameter shows lower values up to 2 months after hatch.