Sodium azide as a specific quencher of singlet oxygen during chemiluminescent detection by luminol and Cypridina luciferin analogues
Sodium azide as a specific quencher of singlet oxygen during chemiluminescent detection by luminol and Cypridina luciferin analogues
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DOI：
10.1002/bio.1296
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发表时间：
2011-11-01
期刊：
影响因子：
2.9
通讯作者：
Bancirova, Martina
中科院分区：
文献类型：
--
作者：
Bancirova, Martina
活性氧（Reactive oxygen species，ROS）在生物体的生理和病理过程中发挥着重要作用。已经研究了各种荧光（CL）化合物，以找到用于检测特定ROS种类的合适的和特异性的探针。已知鲁米诺的CL是非特异性的，并且可以由各种氧化剂诱导。两种Cypridina lactin类似物，CLA和MCLA，已被用于体内ROS的检测。CLA被认为只有在与超氧化物和单线态氧反应时才发光。可以通过使用特定的猝灭剂或清除剂，例如超氧化物歧化酶（SOD）或叠氮化钠（NaN 3）来区分特定的ROS。本文研究了鲁米诺（3-氨基邻苯二甲酰肼）、CLA [2-甲基-6-苯基-3，7-二氢咪唑并[1，2a]吡嗪-3-酮]和MCLA [2-甲基-6-（对甲氧基苯基）-3，7-二氢咪唑并[1，2a]吡嗪-3-酮]在H_2O_2-HRP、H_2O_2-CuSO_4和H_2O_2-NaOCl三种体系中的化学发光反应。在25 ℃下，使用光度计（Fluoroskan Ascent FL和Sirius C），在磷酸盐缓冲液（pH 7.4）中进行测量。使用NaN 3作为单线态氧的特异性猝灭剂。结果表明，对单线态氧的CL的Cypridina Escherin类似物的宣称的特异性进行了讨论。版权所有（C）2011约翰威利父子有限公司
Reactive oxygen species (ROS) are presently thought to play important role in an increasing number of the physiological and pathological processes in living organisms. Various chemiluminescent (CL) compounds have been studied in order to find suitable and specific probes for the detection of particular ROS species. The CL of luminol is known to be non-specific and can be induced by various oxidants. Two Cypridina luciferin analogues, CLA and MCLA, have been used for the detection of ROS in vivo. CLAs are thought to emit light only when reacting with superoxide and singlet oxygen. It is possible to distinguish the particular ROS by using a specific quencher or scavenger, e.g. superoxide dismutase (SOD) or sodium azide (NaN3). The CL reactions of luminol (3-aminophthalhydrazide), CLA [2-methyl-6-phenyl-3,7-dihydroimidazo(1,2a) pyrazin-3-one] and MCLA [2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo(1,2a) pyrazin-3-one] were studied in three hydrogen peroxide decomposition systems (H2O2-HRP; H2O2-CuSO4; and H2O2-NaOCl). The measurements were carried out in phosphate buffer, pH 7.4, at 25 degrees C, using a luminometer (Fluoroskan Ascent FL and Sirius C). NaN3 was used as the specific quencher of singlet oxygen. The results demonstrate that the proclaimed specifity of the CL of Cypridina luciferin analogues towards singlet oxygen has to be discussed. Copyright (C) 2011 John Wiley & Sons, Ltd.