Hydrolysis and biomineralization of porous PLA microspheres and their influence on cell growth
Hydrolysis and biomineralization of porous PLA microspheres and their influence on cell growth
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多孔PLA微球的水解和生物矿化及其对细胞生长的影响
DOI：
10.1016/j.colsurfb.2010.11.016
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发表时间：
2011-06-15
影响因子：
5.8
通讯作者：
Gan, Zhihua
中科院分区：
文献类型：
--
作者：
Shi, Xudong;Jiang, Jian;Gan, Zhihua
聚乳酸（PLA）微球在骨组织工程中具有巨大的应用潜力。然而，它们的应用受到表面和本体性质的限制，例如疏水性、缺乏细胞识别位点和酸性降解产物。磷灰石是一种能有效促进骨细胞粘附和生长的矿物质。在这项研究中，骨样矿物，碳酸磷灰石，成功地用于功能化多孔聚乳酸微球的仿生矿化方法。为了促进磷灰石的形成，首先将多孔聚乳酸微球在氢氧化钠溶液中选择性水解，以增加表面极性阴离子基团的密度，然后浸入模拟体液中进行生物矿化。对原微球和水解微球表面生长的生物活性矿物的形态、组成和相结构进行了定量分析和比较。结果表明，聚乳酸微球表面的水解促进了磷灰石的成核和生长。MG-63细胞在矿化的聚乳酸微球上贴壁良好，铺展活跃，显示了其在骨组织工程中的强大潜力。(C)2010 Elsevier B. V.保留所有权利。
Poly(lactic acid) (PLA) microspheres have great potential in bone tissue engineering. However, their applications have been limited by surface and bulk properties such as hydrophobicity, lack of cell recognition sites and acidic degradation products. Apatite is a mineral which can effectively promote the adhesion and growth of bone cells. In this study, the bonelike mineral, carbonate apatite, was successfully used to functionalize porous PLA microspheres by a biomimetic mineralization method. To improve apatite formation, porous PLA microspheres were first selectively hydrolyzed in NaOH solution to increase the density of polar anionic groups on the surface, and then immersed in simulated body fluid for biomineralization. The morphology, composition, and phase structure of bioactive mineral grown on the original and hydrolyzed PLA microspheres were analyzed and compared quantitatively. The results showed that the hydrolysis which took place on the PLA microspheres enhanced the nucleation and growth of apatite. MG-63 cells attached well and spread actively on the mineralized PLA microspheres, indicating their strong potential in bone tissue engineering. (C) 2010 Elsevier B.V. All rights reserved.