Quality of synthetic hexaploid wheat containing null alleles at Glu-A1 and Glu-B1 loci
Quality of synthetic hexaploid wheat containing null alleles at Glu-A1 and Glu-B1 loci
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含有 Glu-A1 和 Glu-B1 位点无效等位基因的合成六倍体小麦的品质
DOI：
10.1007/s12041-013-0258-7
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发表时间：
2013-08-01
影响因子：
1.5
通讯作者：
Yan, Zehong
中科院分区：
文献类型：
--
作者：
Hu, Xinkun;Dai, Shoufen;Yan, Zehong
膨胀小麦在前期的研究中，双子座植物PI 94668和PI 349045在Glu-A1和Glu-B1位点上均存在无效等位基因。对两份材料中各自的HMW-GS基因Ax、Bx、Ay和By的测序表明，在开放阅读框中具有1至4个读框内终止密码子引起的基因沉默的DNA长度相等。以粗山羊草（Aegilops tauschii）的6个品系为材料，通过未减数配子的自发染色体加倍，获得了6个人工合成的六倍体小麦品系。结果表明，这些双倍体具有Glu-D1的Dx3.1t + Dy 11 *t、Dx2.1t +10 t和Dx 2 t + Dy 12 t三种不同的HMW-GS，而Glu-A1和Glu-B1的HMW-GS均为双缺失。 双子座亲本由于缺乏HMW-GS而非常低。然而，从Ae.在6个人工合成的六倍体小麦品系中，tauschii显著提高了大多数品质相关参数。讨论了这些品系在改良小麦品质方面的潜在价值。
Triticum turgidum ssp. dicoccon PI94668 and PI349045 were identified as containing null alleles at Glu-A1 and Glu-B1 loci in previous investigation. Sequencing of the respective HMW-GS genes Ax, Bx, Ay and By in both accessions indicated equal DNA lengths with gene silencing caused by 1 to 4 in-frame stop codon(s) in the open reading frames. Six synthetic hexaploid wheat lines were produced by crossing PI94668 or PI349045 with six Aegilops tauschii by spontaneous chromosome doubling of unreduced gametes. As expected, these amphiploids had three different HMW-GS: Dx 3.1t + Dy11*t, Dx2.1t + 10t and Dx2t + Dy12t in Glu-D1 but double nulls in Glu-A1 and Glu-B1. dicoccon parents were very low due to the lack of HMW-GSs. However, incorporation of HMW-GS from Ae. tauschii in six synthetic hexaploid wheat lines significantly increased most quality related parameters. The potential values of these wheat lines in improving the quality of wheat are discussed.