Prosystemin Overexpression in Tomato Enhances Resistance to Different Biotic Stresses by Activating Genes of Multiple Signaling Pathways.
Prosystemin Overexpression in Tomato Enhances Resistance to Different Biotic Stresses by Activating Genes of Multiple Signaling Pathways.
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DOI：
10.1007/s11105-014-0834-x
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发表时间：
2015
影响因子：
2.1
通讯作者：
Rao R
中科院分区：
文献类型：
--
作者：
Coppola M;Corrado G;Coppola V;Cascone P;Martinelli R;Digilio MC;Pennacchio F;Rao R
系统素是一种信号肽，可促进番茄对受伤和食草动物攻击的反应。这种 18 个氨基酸的肽是从较大的前体——前系统蛋白中释放出来的。为了研究系统素作为防御信号调节剂的作用，我们培育了过度表达前系统素 cDNA 的番茄 (Solanum lycopersicum) 转基因植物。我们进行了转录组分析，将两种不同的转基因事件与未转化的对照进行比较。 503个差异表达基因的基因本体类别表明多种生物学功能受到影响。系统素促进一系列依赖于不同信号通路的防御基因的表达，并下调与碳固定和碳水化合物代谢相关的基因。这些改变与通常与病原体防御或非生物胁迫保护相关的反应程序存在一定程度的重叠，这意味着系统素信号通路的最终产物可能比预期更加多样化。我们还表明，观察到的转录修饰具有相关的功能结果，因为转基因品系对不同的生物应激源具有更强的抵抗力，例如蚜虫（Macrosiphum euphorbiae）、植物病原真菌（Botrytis cinerea和Alternaria alternata）和植食性幼虫（Spodoptera littoralis）。我们的工作表明，在番茄中，单个基因的调节足以通过增强内源防御途径来提供广泛的抗应激能力。总体而言，数据提供了证据，表明系统素肽可能充当番茄中的 DAMP 信号，充当组织完整性的广泛指标。本文的在线版本 (doi:10.1007/s11105-014-0834-x) 包含补充材料，可供授权用户使用。
Systemin is a signal peptide that promotes the response to wounding and herbivore attack in tomato. This 18-amino acid peptide is released from a larger precursor, prosystemin. To study the role of systemin as a modulator of defense signaling, we generated tomato (Solanum lycopersicum) transgenic plants that overexpress the prosystemin cDNA. We carried out a transcriptomic analysis comparing two different transgenic events with the untransformed control. The Gene Ontology categories of the 503 differentially expressed genes indicated that several biological functions were affected. Systemin promotes the expression of an array of defense genes that are dependent on different signaling pathways and it downregulates genes connected with carbon fixation and carbohydrate metabolism. These alterations present a degree of overlap with the response programs that are classically associated to pathogen defense or abiotic stress protection, implying that end products of the systemin signaling pathway may be more diverse than expected. We show also that the observed transcriptional modifications have a relevant functional outcome, since transgenic lines were more resistant against very different biotic stressors such as aphids (Macrosiphum euphorbiae), phytopathogenic fungi (Botrytis cinerea and Alternaria alternata) and phytophagous larvae (Spodoptera littoralis). Our work demonstrated that in tomato the modulation of a single gene is sufficient to provide a wide resistance against stress by boosting endogenous defense pathways. Overall, the data provided evidence that the systemin peptide might serve as DAMP signal in tomato, acting as a broad indicator of tissue integrity. The online version of this article (doi:10.1007/s11105-014-0834-x) contains supplementary material, which is available to authorized users.