Detection of lymphocytic choriomeningitis virus antibody in colonies of laboratory animals in Japan.
Detection of lymphocytic choriomeningitis virus antibody in colonies of laboratory animals in Japan.
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日本实验动物群体中淋巴细胞性脉络膜脑膜炎病毒抗体的检测。
DOI：
10.1538/expanim1978.35.2_189
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Hironori Miyata
中科院分区：
文献类型：
--
作者：
Hiroshi Sato;Hironori Miyata
应用间接荧光抗体方法检测了日本实验动物群体中的淋巴细胞脉络膜脑膜炎病毒(LCMV)抗体。结果表明，SPF小鼠(3/152，2.2%)和常规小鼠(30/539，5.6%)存在抗体，抗体滴度在1：10~1：160之间。叙利亚仓鼠的抗体阳性率为2.2%(2/89)，黑线姬鼠的抗体阳性率为2.9%(2/68)，日本黑线姬鼠的抗体阳性率为21.4%(3/14)。然而，到目前为止，这种抗体还没有在沙土鼠、Suncus Murinus、豚鼠和大鼠身上表现出来。这些结果表明日本实验动物中存在LCMV感染，并指出了对LCMV进行微生物监测的重要性。
Indirect fluorescent antibody method was applied for a detection of lymphocytic choriomeningitis virus (LCMV) antibody in colonies of laboratory animals in Japan. The results showed that the antibody exist in SPF mice (3/152, 2.2%) and conventional mice (30/539, 5.6%) with the titers ranging from 1:10 to 1:160. The antibody was also detected in 2.2% (2/89) of Syrian hamsters, and 2.9% (2/68) of Apodemus agrarius, 21.4% (3/14) of Japanese harvest mice which have been maintained as laboratory colony for several years. However, the antibody was not demonstrated in Mongolian gerbils, Suncus murinus, guinea pigs and rats, thus far. These results indicate that LCMV infection is present in laboratory animals in Japan, and pointed out the importance of microbiological monitoring for LCMV.