RNA-Seq reveals changes in the Staphylococcus aureus transcriptome following blue light illumination.
RNA-Seq reveals changes in the Staphylococcus aureus transcriptome following blue light illumination.
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DOI：
10.1016/j.gdata.2016.05.011
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发表时间：
2016-09
期刊：
影响因子：
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通讯作者：
Drum BE
中科院分区：
文献类型：
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作者：
Adair TL;Drum BE
为了更好地理解蓝光抑制培养物中金黄色葡萄球菌生长的机制，对金黄色葡萄球菌进行了全转录组分析。使用RNA-Seq进行金黄色葡萄球菌分离株BUSA 2288的基因表达，以分析响应于蓝光暴露的差异基因表达。从S.金黄色葡萄球菌培养物从24个1 ml孔样品汇集，每个样品用250 J/cm 2剂量的465 nm蓝光照射，并从在黑暗中生长的对照培养物汇集。从富集的mRNA样品产生互补DNA文库，并使用Illumina MiSeq Next Generation Sequencer测序。在这里，我们报告了一种类型的分析，确定了32个候选基因进行进一步研究。蓝光已被证明对S.金黄色葡萄球菌，是抗生素耐药生物体的潜在替代疗法。假设细菌灭活的机制与活性氧有关。这些RNA-Seq结果提供了可用于测试这一假设的数据。通过这些实验产生的RNA-Seq数据保存在Gene Expression Omnibus（基因登录号GSE 62055）中，并且可以在NCBI（http：//www.example.com）中找到www.ncbi.nlm.nih.gov/geo/query/acc.cgi? acc= GSE 62055）。
In an effort to better understand the mechanism by which blue light inhibits the growth of Staphylococcus aureus in culture, a whole transcriptome analysis of S. aureus isolate BUSA2288 was performed using RNA-Seq to analyze the differential gene expression in response to blue light exposure. RNA was extracted from S. aureus cultures pooled from 24 1 ml well samples that were each illuminated with a dose of 250 J/cm2 of 465 nm blue light and from control cultures grown in the dark. Complementary DNA libraries were generated from enriched mRNA samples and sequenced using the Illumina MiSeq Next Generation Sequencer. Here we report one type of analysis that identified 32 candidate genes for further investigation. Blue light has been shown to be bactericidal against S. aureus and is a potential alternative therapy for antibiotic resistant organisms. The mechanism for the inactivation of bacteria is hypothesized to involve reactive oxygen species. These RNA-Seq results provide data that may be used to test this hypothesis. The RNA-Seq data generated by these experiments is deposited in Gene Expression Omnibus (Gene accession GSE62055) and may be found at NCBI (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE62055).