Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae
Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae
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DOI：
10.1093/dnares/10.2.67
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发表时间：
2003-04-30
期刊：
影响因子：
4.1
通讯作者：
Kuroiwa, T
中科院分区：
文献类型：
--
作者：
Ohta, N;Matsuzaki, M;Kuroiwa, T
测定了单细胞原始红藻Cyanidioschyzon merolae 10D（Cyanidiophyceae）质体基因组的完整核苷酸序列。基因组是由 149,987 bp 组成的环状 DNA，没有反向重复序列。该质体基因组的G+C含量为37.6%。 C. merolae 质体基因组包含 243 个基因，分布在两条链上，由 36 个 RNA 基因（3 个 rRNA、31 个 tRNA、tmRNA 和一个核糖核酸酶 P RNA 成分）和 207 个蛋白质基因组成，包括未识别的开放阅读框。该基因组的显着特征是基因高度压缩。它的基因间距离非常短（大约 40% 的蛋白质基因重叠）并且没有基因具有内含子。该基因组编码了其他质体基因组中很少发现的几个基因。编码硫酸盐转运蛋白亚基的基因（cysM是第一个在质体基因组中被发现的基因。cys T和cys W基因串联位于C. merolae质体基因组中，它们可能与硫酸盐转运系统的其他核编码成分一起发挥作用。我们的系统发育结果表明，Cyanidiophyceae，包括C. merolae，是红色谱系质体内的基础分支。
The complete nucleotide sequence of the plastid genome of the unicellular primitive red alga Cyanidioschyzon merolae 10D (Cyanidiophyceae) was determined. The genome is a circular DNA composed of 149,987 bp with no inverted repeats. The G + C content of this plastid genome is 37.6%. The C. merolae plastid genome contains 243 genes, which are distributed on both strands and consist of 36 RNA genes (3 rRNAs, 31 tRNAs, tmRNA, and a ribonuclease P RNA component) and 207 protein genes, including unidentified open reading frames. The striking feature of this genome is the high degree of gene compaction; it has very short intergenic distances (approximately 40% of the protein genes were overlapped) and no genes have introns. This genome encodes several genes that are rarely found in other plastid genomes. A gene encoding a subunit of sulfate transporter (cysM is the first to be identified in a plastid genome. The cys T and cys W genes are located in the C. merolae plastid genome in series, and they probably function together with other nuclear-encoded components of the sulfate transport system. Our phylogenetic results suggest that the Cyanidiophyceae, including C. merolae, are a basal clade within the red lineage plastids.