Comparative and Quantitative Global Proteomics Approaches: An Overview.
Comparative and Quantitative Global Proteomics Approaches: An Overview.
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DOI：
10.3390/proteomes1030180
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发表时间：
2013-10-11
期刊：
影响因子：
3.3
通讯作者：
Karamanos Y
中科院分区：
文献类型：
--
作者：
Deracinois B;Flahaut C;Duban-Deweer S;Karamanos Y
蛋白质组学成为研究生物系统的关键工具。两种不同生理状态之间的比较可以揭示生物过程中涉及的细胞和分子机制。蛋白质组学可以通过蛋白质的mRNA含量来确认蛋白质的存在，并提供对细胞中存在的数量的直接测量。可以应用全球的和有针对性的蛋白质组学策略。有针对性的蛋白质组学策略限制了将被监测的特征的数量，然后优化了方法，以获得大量样本的最高灵敏度和吞吐量。全球蛋白质组学策略的优势是，除了样本之间一个或多个蛋白质种类的可测量差异外，不需要任何假设。全球蛋白质组学方法试图从给定的样本中分离、量化和鉴定所有蛋白质。鉴于全球蛋白质组学的比较和定量分析，本综述仅重点介绍了蛋白质和多肽的不同分离和定量技术。将讨论蛋白质的凝胶内和凝胶外定量以及相应的质谱学技术。概述的重点是广泛使用的技术，同时要记住，每种方法都是模块化的，通常可以恢复另一种方法。
Proteomics became a key tool for the study of biological systems. The comparison between two different physiological states allows unravelling the cellular and molecular mechanisms involved in a biological process. Proteomics can confirm the presence of proteins suggested by their mRNA content and provides a direct measure of the quantity present in a cell. Global and targeted proteomics strategies can be applied. Targeted proteomics strategies limit the number of features that will be monitored and then optimise the methods to obtain the highest sensitivity and throughput for a huge amount of samples. The advantage of global proteomics strategies is that no hypothesis is required, other than a measurable difference in one or more protein species between the samples. Global proteomics methods attempt to separate quantify and identify all the proteins from a given sample. This review highlights only the different techniques of separation and quantification of proteins and peptides, in view of a comparative and quantitative global proteomics analysis. The in-gel and off-gel quantification of proteins will be discussed as well as the corresponding mass spectrometry technology. The overview is focused on the widespread techniques while keeping in mind that each approach is modular and often recovers the other.