De novo protein sequence analysis of Macaca mulatta.
De novo protein sequence analysis of Macaca mulatta.
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DOI：
10.1186/1471-2164-8-270
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发表时间：
2007-08-08
期刊：
影响因子：
4.4
通讯作者：
Hemby SE
中科院分区：
文献类型：
--
作者：
Tannu NS;Hemby SE
猕猴是生物医学研究中最常用的非人灵长类动物之一，与人类具有独特的行为，神经解剖学和神经生物化学相似性。这使得它成为一种独特的生物体，可以模拟各种疾病，如精神病和神经退行性疾病，同时还可以深入了解灵长类动物大脑的复杂性。利用恒河猴模型研究人类疾病的一个主要障碍是该物种蛋白质注释的缺乏（约42，000个蛋白质注释），而人类的蛋白质注释为330，210个。缺乏可用的信息限制了使用恒河猴的蛋白质组学规模的研究，在很大程度上依赖于数据库搜索的蛋白质鉴定。虽然使用标准数据库搜索引擎（例如，MASCOT）可以完成，搜索必须使用“广泛的物种数据库”，这并不提供最佳的蛋白质注释的信心。因此，有必要使用手动或自动从头肽序列分析方法来确定部分或完整的氨基酸序列。最近流行的MALDI-TOF-TOF质谱仪产生了复杂的MS/MS裂解模式，难以通过手动从头测序方法在蛋白质组学规模上表征。因此，PEAKS辅助的从头测序进行了猕猴细胞核的胞质蛋白。利用丰度最高的肽片段“b-离子和y-离子”、丰度较低的肽片段“a-离子”以及亚铵离子从MS/MS光谱重新开发可靠和完整的肽序列。生成的序列用于进行同源性搜索以表征蛋白质鉴定。目前的研究验证了一种可靠的方法，可以使用PEAKS从头测序软件从猕猴的不完整序列数据库中自信地表征蛋白质，从而促进了这种动物模型在各种神经蛋白质组学研究中的使用。
Macaca mulatta is one of the most utilized non-human primate species in biomedical research offering unique behavioral, neuroanatomical, and neurobiochemcial similarities to humans. This makes it a unique organism to model various diseases such as psychiatric and neurodegenerative illnesses while also providing insight into the complexities of the primate brain. A major obstacle in utilizing rhesus monkey models for human disease is the paucity of protein annotations for this species (~42,000 protein annotations) compared to 330,210 protein annotations for humans. The lack of available information limits the use of rhesus monkey for proteomic scale studies which rely heavily on database searches for protein identification. While characterization of proteins of interest from Macaca mulatta using the standard database search engines (e.g., MASCOT) can be accomplished, searches must be performed using a 'broad species database' which does not provide optimal confidence in protein annotation. Therefore, it becomes necessary to determine partial or complete amino acid sequences using either manual or automated de novo peptide sequence analysis methods. The recently popularized MALDI-TOF-TOF mass spectrometer yields a complex MS/MS fragmentation pattern difficult to characterize by manual de novo sequencing method on a proteomics scale. Therefore, PEAKS assisted de novo sequencing was performed on nucleus accumbens cytosolic proteins from Macaca mulatta. The most abundant peptide fragments 'b-ions and y-ions', the less abundant peptide fragments 'a-ions' as well as the immonium ions were utilized to develop confident and complete peptide sequences de novo from MS/MS spectra. The generated sequences were used to perform homology searches to characterize the protein identification. The current study validates a robust method to confidently characterize the proteins from an incomplete sequence database of Macaca mulatta, using the PEAKS de novo sequencing software, facilitating the use of this animal model in various neuroproteomics studies.
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影响因子：
7
作者：
Tannu, NS;Rao, VK;Raghow, R
通讯作者：
Raghow, R
影响因子：
2.9
作者：
Getie, M;Schmelzer, CEH;Neubert, RHH
通讯作者：
Neubert, RHH
影响因子：
2.9
作者：
Samyn, Bart;Sergeant, Kjell;Van Beeumen, Jozef
通讯作者：
Van Beeumen, Jozef
DOI：
10.1016/0165-022x(82)90013-6
发表时间：
1982-01-01
影响因子：
--
作者：
BJELLQVIST, B;EK, K;POSTEL, W
通讯作者：
POSTEL, W
影响因子：
56.9
作者：
HUNT, DF;HENDERSON, RA;ENGELHARD, VH
通讯作者：
ENGELHARD, VH