The phylogenetics of triculine snails (Rissooidea:Pomatiopsidae) from south-east Asia and southern China:: Historical biogeography and the transmission of human schistosomiasis
The phylogenetics of triculine snails (Rissooidea:Pomatiopsidae) from south-east Asia and southern China:: Historical biogeography and the transmission of human schistosomiasis
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DOI：
10.1093/mollus/69.3.263
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发表时间：
2003-08-01
影响因子：
1.2
通讯作者：
Southgate, VR
中科院分区：
文献类型：
--
作者：
Attwood, SW;Ambu, S;Southgate, VR
对东南亚和中国西南地区6种石螺的1个核基因（18 SrRNA）和2个线粒体基因（16 SrRNA和CO 1）的部分DNA序列进行了分析。采集了以下分类群的新鲜田间样品：老挝南部的开放新锥虫（拟步甲亚科：Pachydrobiini）;泰国北方的伯氏新锥虫;中国西南部的湖北罗氏钉螺（Pomatiopsinae：Pomatiopsini）;马来西亚西部的罗氏螺属（Pachydrobiini）;泰国北方的博林拟步甲（拟步甲亚科：拟步甲族）;以及中国西南部的霍滕拟步甲。还使用了取自GenBank的Gammatricula fujianensis（Pachydrobiini）的序列。这是首次发表的N. burchi和Robertsiella。除N. burchi，所有这些类群在自然界中传播血吸虫; aperta，奥氏木属O. H. robertsoni和Robertsiella将血吸虫传播给人类。发现所有上述分类群在18 S基因座处均为同源的（即未显示序列变异）。系统发育估计使用一个完全优化的模型的核苷酸取代和最大似然或贝叶斯方法。两种不同方法所得结果一致。16 S树和CO 1树除了G. fujianensis、T. hortensis和其他类群。这些数据证实了N. aperta和N. burchi;这对Pachydrobiini的历史地理模型的选择的影响进行了讨论。
Partial DNA sequences were examined for one nuclear (18S rRNA) and two mitochondrial (16S rRNA and CO1) loci for six species of pomatiopsid snail (Gastropoda:Rissooidea:Pomatiopsidae) from south-east Asia and south-west China. Fresh field samples were collected for the following taxa: Neotricula aperta (Triculinae:Pachydrobiini) from southern Laos; Neotricula burchi from northern Thailand; Oncomelania hupensis robertsoni (Pomatiopsinae:Pomatiopsini) from south-west China; Robertsiella sp. (Pachydrobiini) from West Malaysia; Tricula bollingi (Triculinae:Triculini) from northern Thailand; and Tricula hortensis from south-west China. Sequences taken from GenBank for Gammatricula fujianensis (Pachydrobiini) were also used. This represents the first published DNA sequence data for N. burchi and Robertsiella. With the exception of N. burchi, all of these taxa transmit Schistosoma in nature; N. aperta, O. h. robertsoni and Robertsiella transmit Schistosoma to humans. All of the above taxa were found to be homogenetic (i.e. showed no sequence variation) at the 18S locus. Phylogenies were estimated using a fully optimized model of nucleotide substitution and either a maximum likelihood or Bayesian method. Good congruence was observed between the phylogenies resulting from the two different methods. The 16S and CO1 trees showed the same topology except for the relationships between G. fujianensis, T. hortensis and the other taxa. The data confirmed the congeneric status of N. aperta and N. burchi; the implications of this for the choice of historical biogeographical model for the Pachydrobiini are discussed.