Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3)
Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3)
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DOI：
10.1016/s0014-5793(00)02219-5
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发表时间：
2000-11-24
期刊：
影响因子：
3.5
通讯作者：
Farrow, SN
中科院分区：
文献类型：
--
作者：
Kaptein, A;Jansen, M;Farrow, SN
wsl1 /TRAMP （DR3）是肿瘤坏死因子（TNF）受体超家族的一员，对NF-kappaB活化和凋亡有影响，一种新的TNF相关分子TWEAK被认为是该受体的配体。利用人类和小鼠的TWEAK配体，在体外结合实验中显示，TWEAK和WSL-1/TRAMP不相互作用，并且在细胞表面不表达WSL-1/TRAMP的细胞上强烈结合。在这些细胞中也观察到TWEAK的生物活性。最后，从WSL-1/TRAMP敲除小鼠中分离的细胞显示保留了与TWEAK相互作用的能力。这些结果表明WSL-1/TRAMP不是TWEAK (C) 2000欧洲生化学会联合会的主要受体。Elsevier Science B.V.版权所有。
WSL-1/TRAMP (DR3) is a member of the tumour necrosis factor (TNF) receptor superfamily which exhibits effects on NF-kappaB activation and apoptosis, TWEAK, a novel TNF-related molecule, has been proposed as the ligand for this receptor. Utilising both human and murine TWEAK ligand, it is shown that TWEAK and WSL-1/TRAMP do not interact in an in vitro binding assay and that TWEAK binds strongly to cells that do not express WSL-1/TRAMP on the cell surface. Biological activity of TWEAK is also observed in these cells. Finally, cells isolated from WSL-1/TRAMP knockout mice are shown to retain their ability to interact with TWEAK. These results suggest that WSL-1/TRAMP is not the major receptor for TWEAK (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.