Emx1 is a marker for pyramidal neurons of the cerebral cortex
Emx1 is a marker for pyramidal neurons of the cerebral cortex
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DOI：
10.1093/cercor/11.12.1191
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发表时间：
2001-12-01
期刊：
影响因子：
3.7
通讯作者：
Parnavelas, JG
中科院分区：
文献类型：
--
作者：
Chan, CH;Godinho, LN;Parnavelas, JG
含有同源框的基因Emx 1是果蝇空气门的小鼠同源物，在发育中的端脑皮层中特异性表达。已有研究表明，Emx1转录本及其蛋白产物在大脑皮质的大多数细胞增殖、迁移、分化和成熟过程中均存在。我们在这里提供的证据，在发育中的大鼠的实验方法的基础上，在支持的假设，该基因的表达仅限于锥体神经元。具体来说，我们发现，类似于锥体神经元，表达Emx 1的细胞分布在所有皮层层，除了第一层。应用原位杂交和免疫细胞化学技术，在光镜和电镜水平上观察到这些细胞的密度、分布、索马形态和超微结构特征与锥体神经元相同。双标记实验证实，绝大多数表达Emx 1的细胞也含有谷氨酸，这是锥体神经元的标志物。我们还发现，这种基因在分离的皮质细胞培养物中的大多数含谷氨酸的神经元和嵌合小鼠皮质中放射状排列的锥体细胞克隆中的绝大多数细胞中表达。因此，同源异型盒基因Emx 1可以可靠地用作锥体细胞谱系的标记。
The homeobox-containing gene, Emx1, a mouse homologue of Drosophila empty spiracles, is specifically expressed in the developing telencephalic cortex. It has been reported that Emx1 transcripts and the protein product are localized in most cells of the cerebral cortex during the process of proliferation, migration, differentiation and maturation. We provide evidence here, based on a multitude of experimental approaches in developing rats, in support of the hypothesis that the expression of this gene is restricted to pyramidal neurons. Specifically, we found that, similar to pyramidal neurons, cells expressing Emx1 are distributed in all cortical layers, except layer I. Using in situ hybridization and immunocytochemistry at the light and electron microscope levels, we have shown that the density, distribution, soma shape and ultrastructural features of these cells were identical to those of pyramidal neurons. Double-labelling experiments confirmed that the vast majority of Emx1-expressing cells also contained glutamate, a marker of pyramidal neurons. We also found that this gone is expressed by most glutamate-containing neurons in dissociated cortical cell cultures and the vast majority of cells in radially arranged clones of pyramidal cells in the cortices of chimeric mice. Thus, the homeobox gene Emx1 can be reliably used as a marker of the pyramidal cell lineage.