Molecular cloning and characterisation of the ribC gene from Bacillus subtilis: A point mutation in ribC results in riboflavin overproduction
Molecular cloning and characterisation of the ribC gene from Bacillus subtilis: A point mutation in ribC results in riboflavin overproduction
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DOI：
10.1007/s004380050393
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发表时间：
1997-03-18
期刊：
影响因子：
--
通讯作者：
Hohmann, HP
中科院分区：
文献类型：
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作者：
Coquard, D;Huecas, M;Hohmann, HP
在过量生产核黄素的枯草芽孢杆菌菌株RB52和RB50的基因组147度位置定位了一个导致玫瑰黄素抗性和核黄素生物合成失控的突变。染色体定位表明RB52和RB50的失调突变是先前鉴定的ribC突变的等位基因。我们克隆了ribC基因，发现它编码一个假定的36kda蛋白。令人惊讶的是，RibC与其他各种细菌的黄素激酶和FAD合成酶具有显著的序列相似性。通过比较RB50野生型亲本菌株的RibC氨基酸序列与RB50过表达核黄素的突变株的RibC序列，我们发现了一个点突变，导致产物c末端的Gly与Ser交换。
A mutation leading to roseoflavin resistance and deregulated riboflavin biosynthesis was mapped in the genome of the riboflavin-overproducing Bacillus subtilis strains RB52 and RB50 at map position 147 degrees. The chromosomal location indicates that the deregulating mutation in RB52 and RB50 is an allele of the previously identified ribC mutation. We cloned the ribC gene and found that it encodes a putative 36-kDa protein. Surprisingly, RibC has significant sequence similarity to flavin kinases and FAD synthases from various other bacterial species. By comparing the deduced amino acid sequence of RibC from the wild-type parent strain of RB50 with the RibC sequence from the riboflavin-overexpressing RB50 mutant we identified a point mutation that resulted in a Gly to Ser exchange in the C-terminal region of the product.