Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme.
Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme.
复制标题
氨基酸和甘氨酸乙酯作为溶菌酶的新型结晶试剂。
DOI：
10.1107/s174430911001376x
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
H. Yamaguchi
中科院分区：
文献类型：
--
作者：
L. Ito;K. Shiraki;H. Yamaguchi
几种氨基酸及其衍生物是蛋白质化学领域的重要添加剂。以鸡蛋清溶菌酶为模型，研究了带电荷氨基酸和甘氨酸乙酯作为沉淀剂在蛋白质结晶过程中的作用。讨论了用这些试剂作沉淀剂的HEWL结晶。
Several amino acids and their derivatives are prominent additives in the field of protein chemistry. This study reports the use of charged amino acids and glycine ethyl ester as precipitants in protein crystallization, using hen egg-white lysozyme (HEWL) as a model. A discussion of the crystallization of HEWL using these reagents as precipitating agents is given.