CCR4 and CCR5 expression in conjunctival specimens as differential markers of TH1/TH2 in ocular surface disorders
CCR4 and CCR5 expression in conjunctival specimens as differential markers of TH1/TH2 in ocular surface disorders
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DOI：
10.1016/j.jaci.2005.05.033
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发表时间：
2005-09-01
影响因子：
14.2
通讯作者：
Brignole-Baudouin, F
中科院分区：
文献类型：
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作者：
Baudouin, C;Liang, H;Brignole-Baudouin, F
背景：慢性眼表疾病（OSDs）的准确炎症机制无法常规评估。研究眼表炎症通路的新技术具有重要意义。目的：探讨慢性阻塞性肺疾病患者结膜印迹细胞学标本中分别与T(H)2和T(H)1系统相关的CCR4和CCR5以及HLA-DR的表达情况。方法：在本病例对照研究中，选取春性角膜结膜炎（n = 21）、巨大乳头状结膜炎（n = 6）、干枯性角膜结膜炎（KCS; n = 17）或接受局部抗青光眼治疗（n = 31）的患者和20名正常受试者的印象细胞学标本。结膜细胞与CCR4、CCR5、CD45和HLA-DR的单克隆抗体孵育，使用流式细胞术以隐蔽的方式定量结膜炎症。结果：青光眼组和KCS组HLA-DR高于过敏组和正常组。CCR4在过敏组和青光眼组中过表达，而CCR5在KCS组和青光眼组中较高。CD45在所有组中仅被少数细胞表达，几乎没有显著差异。CCR4表达与CCR5、HLA-DR呈负相关，而CCR5表达与HLA-DR呈正相关。结论：本研究证实了OSDs结膜上皮中趋化因子受体的过表达。CCR4和CCR5的表达可能根据所涉及的免疫途径而变化。眼表炎症反应的准确机制-即与T(H)1或T(H)2系统相关的机制-可以通过CCR4/CCR5谱来区分。我们的研究结果还表明，长期使用局部治疗可能会刺激这两个系统。
Background: Accurate inflammatory mechanisms in chronic ocular surface diseases (OSDs) cannot routinely be assessed. New techniques for investigating ocular surface inflammatory pathways are of major importance.Objective: To investigate the expressions of CCR4 and CCR5, known to be related to the T(H)2 and T(H)1 systems, respectively, and HLA-DR in conjunctival impression cytology specimens from patients with chronic OSDs.Methods: In this case-controlled study, impression cytology specimens were taken in a series of patients with vernal keratoconjunctivitis (n = 21), giant papillary conjunctivitis (n = 6), or keratoconjunctivitis sicca (KCS; n = 17), or receiving topical antiglaucoma treatments (n = 31), and from 20 normal subjects. Conjunctival cells were incubated with mAbs to CCR4, CCR5, CD45, and HLA-DR to quantify conjunctival inflammation in a masked manner using flow cytometry.Results: HLA-DR was higher in the glaucoma and KCS groups than in allergic and normal eyes. CCR4 was overexpressed in allergy and glaucoma, whereas CCR5 was higher in the KCS and glaucomatous groups. CD45 was expressed by only few cells in all groups, with almost no significant differences. CCR4 expression was negatively correlated with CCR5 and HLA-DR, whereas CCR5 was positively correlated with HLA-DR.Conclusion: This study confirms the overexpression of chemokine receptors by the conjunctival epithelium in OSDs. CCR4 and CCR5 expression may vary according to the immune pathway involved. Accurate mechanisms in ocular surface inflammatory reactions-that is, those related to the T(H)1 or T(H)2 systems-could be differentiated by CCR4/CCR5 profiles. Our results also suggest that long-term use of topical treatments may stimulate both systems.