Nicotine-Mediated ADP to Spike Transition: Double Spiking in Septal Neurons.
Nicotine-Mediated ADP to Spike Transition: Double Spiking in Septal Neurons.
复制标题
DOI：
10.1007/s00232-015-9853-2
复制
发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Colom L
中科院分区：
文献类型：
--
作者：
Kodirov SA;Wehrmeister M;Colom L
外侧隔（LS）的大多数神经元在静息膜电位下是电沉默的。尼古丁会短暂兴奋一部分神经元，偶尔会在长时间应用后导致持久的爆发活动。我们观察到尼古丁存在下自发动作电位（AP）的频率和振幅的同时变化。在长时间的暴露过程中，尼古丁增加了爆发内的尖峰数量。尼古丁效应的标志之一是出现双峰值（也称为爆发）。连续应用尼古丁后触发的51个自发峰的排列显示，后去极化电位的斜率逐渐增加（1.4 vs. 3 mV/ms），神经元发射第二个AP，称为双峰。从单AP到双棘波的过渡增加了后超极化电位的幅度。第二个（过早）AP的幅度比第一个小，这种相关性在持续时间（半宽度）方面持续存在。据我们所知，在尼古丁的存在下，在一般的隔结构中，特别是在LS中，以前没有报道过类似的爆发活动。
The majority of neurons in lateral septum (LS) are electrically silent at resting membrane potential. Nicotine transiently excites a subset of neurons and occasionally leads to long lasting bursting activity upon longer applications. We have observed simultaneous changes in frequencies and amplitudes of spontaneous action potentials (AP) in the presence of nicotine. During the prolonged exposure, nicotine increased numbers of spikes within a burst. One of the hallmarks of nicotine effects was the occurrences of double spikes (known also as bursting). Alignment of 51 spontaneous spikes, triggered upon continuous application of nicotine, revealed that the slope of after-depolarizing potential gradually increased (1.4 vs. 3 mV/ms) and neuron fired the second AP, termed as double spiking. A transition from a single AP to double spikes increased the amplitude of after-hyperpolarizing potential. The amplitude of the second (premature) AP was smaller compared to the first one, and this correlation persisted in regard to their duration (half-width). A similar bursting activity in the presence of nicotine, to our knowledge, has not been reported previously in the septal structure in general and in LS in particular.