Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria
Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria
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DOI：
10.1186/1476-0711-9-1
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发表时间：
2010-01-01
影响因子：
5.7
通讯作者：
Vahaboglu, Haluk
中科院分区：
文献类型：
--
作者：
Kasap, Murat;Fashae, Kayode;Vahaboglu, Haluk
背景：我们研究了大肠埃希菌的β-内酰胺酶。产气单胞菌分离物从一名两岁患者的血液中回收。该分离株表现出典型的盘扩散表型的AmpC-ESBL coproducer.Methods：微生物学研究进行了根据标准协议。结果：转接合法仅能传递小质粒上编码的一种窄谱β-内酰胺酶（TEM-1）。克隆了ESBL基因，并在E. coli宿主。重组质粒的序列分析表明，插入序列为IS 26的重组质粒含有blaSHV-12。结论：本研究首次证实了SHV-12在尼日利亚的存在。
Background: We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.Methods: Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.Results: By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26.Conclusion: This is the first study demonstrated the occurrence of SHV-12 in Nigeria.