Germ line activation of the Tie2 and SMMHC promoters causes noncell-specific deletion of floxed alleles
Germ line activation of the Tie2 and SMMHC promoters causes noncell-specific deletion of floxed alleles
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DOI：
10.1152/physiolgenomics.90284.2008
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发表时间：
2008-09-01
影响因子：
4.6
通讯作者：
Sigmund, Curt D.
中科院分区：
文献类型：
--
作者：
de Lange, Willem J.;Halabi, Carmen M.;Sigmund, Curt D.
de Lange WJ，Halabi CM，Beyer AM，Sigmund CD. Tie 2和SMMHC启动子的种系激活导致floxed等位基因的非细胞特异性缺失。Physiol Genomics 35：1-4，2008.首次发表于2008年7月8日; doi：10.1152/physiolgenomics.90284.2008。通过Cre-loXP重组产生的组织特异性敲除已成为操纵小鼠基因组的重要工具。通常，进行两轮连续的育种以产生携带两个floxed靶基因等位基因和组织特异性表达Cre重组酶的转基因的小鼠。我们在此表明，两个启动子通常用于产生内皮特异性（Tie 2）和平滑肌特异性[平滑肌肌球蛋白重链（Smmhc）]敲除小鼠表现出活性的女性和男性生殖系，分别。这可能导致在第二代中非组织特异性的无效等位基因的遗传。因此，需要仔细的实验设计，以确保组织特异性敲除确实是组织特异性的，并且使用适当的对照来比较菌株。
de Lange WJ, Halabi CM, Beyer AM, Sigmund CD. Germ line activation of the Tie2 and SMMHC promoters causes noncell-specific deletion of floxed alleles. Physiol Genomics 35: 1-4, 2008. First published July 8, 2008; doi: 10.1152/physiolgenomics.90284.2008.-Tissue-specific knockouts generated through Cre-loxP recombination have become an important tool to manipulate the mouse genome. Normally, two successive rounds of breeding are performed to generate mice carrying two floxed target-gene alleles and a transgene expressing Cre-recombinase tissue-specifically. We show herein that two promoters commonly used to generate endothelium-specific (Tie2) and smooth muscle-specific [smooth muscle myosin heavy chain (Smmhc)] knockout mice exhibit activity in the female and male germ lines, respectively. This can result in the inheritance of a null allele in the second generation that is not tissue specific. Careful experimental design is required therefore to ensure that tissue-specific knockouts are indeed tissue specific and that appropriate controls are used to compare strains.