Equilibrium sedimentation of influenza virus in caesium chloride density gradients.
Equilibrium sedimentation of influenza virus in caesium chloride density gradients.
复制标题
流感病毒在氯化铯密度梯度中的平衡沉降。
DOI：
10.1038/icb.1960.55
复制
发表时间：
1960
期刊：
影响因子：
--
通讯作者：
R. Barry
中科院分区：
文献类型：
--
作者：
R. Barry
标准和不完整甲型流感病毒的制备物在氯化铯溶液的密度梯度中进行平衡沉降。氯化铯浓缩溶液迅速破坏了流感病毒的感染性。对于标准病毒制剂，血凝素在这些梯度中的分布表明它们主要由密度相似的颗粒和一小类密度较低的颗粒组成。所测试的两种菌株中该类的大小不同。一种菌株获得的电子显微镜可见颗粒的分布与血凝素的分布相对应。没有证据表明标准制剂中存在单独类别的无活性颗粒。病毒酶均匀分布在病毒群体中。不完整的病毒制剂缺乏标准病毒制剂的同质性。随着不完整性的增加，一系列密度不同的粒子类型出现。
Preparations of standard and incomplete influenza A virus were subjected to equilibrium sedimentation in density gradients of caesium chloride solutions. Concentrated solutions of caesium chloride rapidly destroyed the infectivity of influenza virus.For standard virus preparations, the distribution of haemagglutinin in these gradients indicates that they consist largely of particles of similar density with a small class of particles of lower density. The size of this class was different in the two strains tested. The distribution of electron‐microscopically visible particles obtained for one strain corresponded to the distribution of haemagglutinin. There was no evidence that a separate class of inactive particles exists in standard preparations.The viral enzyme is uniformly distributed in the virus population.Incomplete virus preparations lack the homogeneity of standard virus preparations. As incompleteness increases, an array of particle types of variably lower density appears.