Interactions of antithrombin and proteins in the plasma contact activation system with immobilized functional heparin
Interactions of antithrombin and proteins in the plasma contact activation system with immobilized functional heparin
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DOI：
10.1002/jbm.a.10118
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发表时间：
2003-11-01
影响因子：
4.9
通讯作者：
Brash, JL
中科院分区：
文献类型：
--
作者：
Cornelius, RM;Sanchez, J;Brash, JL
报道了抗凝血酶(AT)和接触相凝血因子与两种商业肝素化表面的相互作用。将Carmeda(CBAS)和Corline表面以及对照组(磺化聚乙烯表面和CBAS类似物，其中使用的肝素没有特定的AT结合序列)暴露于人血浆中。吸附的蛋白质用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和免疫印迹法检测。CBAS和Corline表面吸附了大量的AT，而对照表面的吸附可以忽略不计。四种接触相凝血因子的免疫印迹显示，CBAS和Corline表面的接触激活程度低于对照组。用FXA抑制实验测定了吸附的功能AT，结果表明CBAS表面吸附的AT大约是Corline表面的4倍。AT在对照表面的吸附很小。基于激肽释放酶生成的FXII和FXIIa吸附分析表明，四种表面吸附的FXII量相同。然而，在对照组上，FXII大部分处于激活状态，而在CBAS和Corline表面上几乎没有激活。(C)2003年威利期刊公司。
The interactions of antithrombin (AT) and the contact phase clotting factors with two commercially available heparinized surfaces are reported. The Carmeda (CBAS) and Corline surfaces along with controls (a sulfonated polyethylene surface and a CBAS analog in which the heparin used was devoid of specific AT-binding sequences) were exposed to human plasma. Adsorbed proteins were eluted and examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The CBAS and Corline surfaces adsorbed large amounts of AT, whereas adsorption on the controls was negligible. Immunoblots for the four contact phase clotting factors indicated less contact activation on the CBAS and Corline surfaces than on the controls. Determination of adsorbed functional AT using a FXa inhibition assay showed that the CBAS surface adsorbed about 4 times as much AT as the Corline surface. Adsorption of AT to the control surfaces was minimal. Assays for adsorbed FXII and FXIIa based on kallikrein generation showed that all four surfaces adsorbed similar amounts of FXII. However, on the controls, most of the FXII was in activated form, whereas on the CBAS and Corline surfaces very little activation occurred. (C) 2003 Wiley Periodicals, Inc.