REVERSAL OF NATURAL KILLING SUSCEPTIBILITY IN TARGET-CELLS EXPRESSING TRANSFECTED CLASS-I HLA GENES
REVERSAL OF NATURAL KILLING SUSCEPTIBILITY IN TARGET-CELLS EXPRESSING TRANSFECTED CLASS-I HLA GENES
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DOI：
10.1073/pnas.86.7.2361
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发表时间：
1989-04-01
影响因子：
11.1
通讯作者：
CRESSWELL, P
中科院分区：
文献类型：
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作者：
STORKUS, WJ;ALEXANDER, J;CRESSWELL, P
许多研究表明，靶细胞对自然杀伤（NK）的抵抗力可能与其主要组织相容性复合物（MHC）I类抗原的表达水平相关。为了直接检验这一假设，用 MHC I 类基因转染 NK 敏感的 I 类缺陷人类 B 细胞系。转染的 HLA（而非 H-2）I 类基因产物的表达导致对人 NK 介导的接合和细胞溶解的敏感性丧失。此外，这种保护并不影响白细胞介素2刺激的人NK效应细胞介导的细胞毒性。
A number of studies have suggested that resistance of target cells to natural killing (NK) may be correlated with their level of expression of major histocompatibility complex (MHC) class I antigens. To examine this hypothesis directly, a NK-sensitive class I-deficient human B-cell line was transfected with MHC class I genes. The expression of transfected HLA, but not H-2, class I gene products resulted in loss of susceptibility to human NK-mediated conjugation and cytolysis. Furthermore, this protection did not extent to cytotoxicity mediated by interleukin 2-stimulated human NK effector cells.