Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives
Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives
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DOI：
10.1111/j.1600-0722.2006.00342.x
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发表时间：
2006-04-01
影响因子：
1.9
通讯作者：
Pashley, DH
中科院分区：
文献类型：
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作者：
Nishitani, Y;Yoshiyama, M;Pashley, DH
已知温和酸可激活牙本质基质金属蛋白酶（MMPs）。所有自酸蚀牙科粘合剂都是酸性的（pH 1.5-2.7），并且可以激活牙本质MMPs。本研究的目的是比较几种多功能粘合剂在粉末状矿化牙本质中激活明胶溶解和胶原溶解活性的能力。将由人牙齿制成的粉末状牙本质与多合一粘合剂（Clearfil Tri-S Bond、G-Bond、Adper Prompt L-Pop）或自蚀刻底漆（Clearfil SE Bond底漆）混合不同的时间，然后通过使用丙酮提取粘合剂来终止反应。新鲜的未经处理的矿化牙本质粉末的明胶分解活性为3.31 +/- 0.39相对荧光单位（RFU）/mg干重（24 h），随着储存时间的推移，在6-8周后增加到87.5 RFU mg（-1）（24 h）。当新鲜粉末用酸性Tri-S键处理时，明胶分解活性在20 min后从3.24 +/- 0.70 RFU mg（-1）增加到> 112.5 RFU mg（-1）（24 h），然后保持不变。具有较低pH值的单体产生较少活性。有一个显着的，直接的明胶分解活性和pH值之间的相关性，与三S给予最高的活动。用Tri-S树脂涂覆牙本质粉末阻止荧光底物接近酶，即使它激活了酶。总之，自酸蚀粘合剂可能会激活潜在的MMP，并将活性提高到接近最大水平，并随着时间的推移导致树脂-牙本质粘结的降解。
Mild acids are known to activate dentin matrix metalloproteinase (MMPs). All self-etching dental adhesives are acidic (pH 1.5-2.7) and may activate dentin MMPs. The purpose of this study was to compare the ability of several all-in-one adhesives to activate gelatinolytic and collagenolytic activities in powdered mineralized dentin. Powdered dentin made from human teeth was mixed with all-in-one adhesives (Clearfil Tri-S Bond, G-Bond, Adper Prompt L-Pop) or a self-etching primer (Clearfil SE Bond primer) for varying times and then the reaction was stopped by extracting the adhesives using acetone. Fresh untreated mineralized dentin powder had a gelatinolytic activity of 3.31 +/- 0.39 relative fluorescent units (RFU) per mg dry weight (24 h) that increased, over storage time, to 87.5 RFU mg(-1) (24 h) after 6-8 wk. When fresh powder was treated with acidic Tri-S Bond, the gelatinolytic activity increased from 3.24 +/- 0.70 RFU mg(-1) to > 112.5 RFU mg(-1) (24 h) after 20 min and then remained unchanged. Monomers with lower pH values produced less activity. There was a significant, direct correlation between gelatinolytic activity and pH, with Tri-S giving the highest activity. Coating dentin powder with Tri-S resin prevented fluorescent substrates from gaining access to the enzyme, even though it activated the enzyme. In conclusion, self-etch adhesives may activate latent MMP and increase the activity to near-maximum levels and contribute to the degradation of resin-dentin bonds over time.