High plasma level of S100A8/S100A9 and S100A12 at admission indicates a higher risk of death in septic shock patients
High plasma level of S100A8/S100A9 and S100A12 at admission indicates a higher risk of death in septic shock patients
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DOI：
10.1038/s41598-019-52184-8
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发表时间：
2019-10-30
影响因子：
4.6
通讯作者：
Payen, Didier
中科院分区：
文献类型：
--
作者：
Dubois, Christelle;Marce, Dominique;Payen, Didier
脓毒症的严重程度，预测结果或器官功能障碍的可逆性的生物标志物是必要的。入院时血浆DAMP水平的测量可以反映脓毒性休克中细胞损伤的严重程度，这可能预测预后并降低用昂贵的治疗方法过度治疗患者的风险。我们在感染性休克患者入院的前24小时内测定了两种DAMP，S100 A8/S100 A9和S100 A12的血浆水平。从我们的脓毒症数据库中选择了49例SOFA评分相似的脓毒性休克患者，以比较幸存者和非幸存者的相似比例。使用内部ELISA将S100 A8/S100 A9和S100 A12的血浆水平与健康志愿者进行比较。S100 A8/S100 A9和S100 A12的血浆水平感染性休克患者血清中的β-半胱氨酸（5.71 [2.60-13.63] μ g/mL）和β-半胱氨酸（0.48 [0.22-1.05] μ g/mL）均高于健康志愿者（1.18 [0.74-1.93] μ g/mL和0.09 [0.02-0.39] μ g/mL）（P < 0.0001和P = 0.0030）。第28天非存活者中S100 A8/S100 A9和S100 A12的水平（11.70 [2.85-24.36] μ g/mL和0.62 [0.30-1.64] μ g/mL）显著高于存活者（4.59 [2.16-7.47] μ g/mL和0.30 [0.20-0.49] μ g/mL）（P = 0.0420和P = 0.0248）且相关性良好（斯皮尔曼r = 0.879，P < 0.0001）。感染性休克患者入院时血浆钙颗粒蛋白水平较高，死亡者高于存活者。当SOFA评分相似时，这些标志物可能表明死亡风险较高，并有助于对患者进行分层，以改善护理和治疗选择。
Biomarkers in sepsis for severity, prediction of outcome or reversibility of organ dysfunction are warranted. Measurements of plasma DAMP levels at admission can reflect the severity of cellular damage in septic shock, which might predict the prognosis and reduce the risk of overtreating patients with costly therapies. We measured plasma levels of two DAMPs, S100A8/S100A9 and S100A12 during the first 24 h of admission of septic shock patients. Forty-nine septic shock patients with a similar SOFA scores were selected from our sepsis database to compare a similar proportion of survivors and non-survivors. Plasma levels of S100A8/S100A9 and S100A12 were compared with healthy volunteers using in-house ELISA. Plasma levels of S100A8/S100A9 and S100A12 (5.71 [2.60-13.63] mu g/mL and 0.48 [0.22-1.05] mu g/mL) were higher in septic shock patients than in healthy volunteers (1.18 [0.74-1.93] mu g/mL and 0.09 [0.02-0.39] mu g/mL) (P < 0.0001 and P = 0.0030). Levels of S100A8/S100A9 and S100A12 in non-survivors at day 28 (11.70 [2.85-24.36] mu g/mL and 0.62 [0.30-1.64] mu g/mL) were significantly higher than in survivors (4.59 [2.16-7.47] mu g/mL and 0.30 [0.20-0.49] mu g/mL) (P = 0.0420 and P = 0.0248) and correlated well (Spearman r = 0.879, P < 0.0001). The high level of plasma calgranulins at admission in septic shock, were higher in non-survivors compared to survivors. These markers could indicate a higher risk of death when SOFA scores are similar and help the stratification of patients for improved care and therapy selection.