Evaluation of Statens Serum Institut enteric medium for detection of enteric pathogens
Evaluation of Statens Serum Institut enteric medium for detection of enteric pathogens
复制标题
DOI：
10.1128/jcm.37.7.2312-2316.1999
复制
发表时间：
1999-07-01
影响因子：
9.4
通讯作者：
Espersen, F
中科院分区：
文献类型：
--
作者：
Blom, M;Meyer, A;Espersen, F
评价了Statens Serum Institut（SSI）肠道培养基用于分离和直接鉴定肠道病原体的有效性。可以通过使用SSI肠介质读取S种不同的生化反应，从而允许直接鉴定一系列肠道病原体。测试的所有248种革兰氏阴性细菌物种在SSI肠介质上生长。248种细菌中只有10种（4%）在生化反应中显示出不一致的结果，并且这些细菌都不是肠道病原体。43的47个肠道病原体（92%）的SSI肠介质和5%的血琼脂产生相同的半定量增长率，而三个弧菌属，和一个气单胞菌属，表现出减少的增长。革兰氏阳性菌在SSI肠道培养基上不生长。大多数肠道病原体的检测限为每毫升粪便50个细菌，但需要更多数量的弧菌属和一些志贺氏菌属进行检测。比较了125种肠道致病菌和12种耶尔森氏菌在SSI、木糖赖氨酸脱氧胆酸盐（SLD）、Hektoen肠溶菌（HE）、沙门氏菌-志贺菌（SS）和头孢磺啶-伊加生-新生霉素（CIN）琼脂上的生长速度。应用200 CFU后，SSI、SLD、HE和SS琼脂的检出率分别为99%、92%、88%和82%。12种耶尔森菌在SSI肠培养基和CIN琼脂上均生长良好。我们认为SSI肠培养基的性能优于其他试验培养基。在培养基上直接鉴定肠道病原体也可以提供更快速的诊断。
The efficacy of the Statens Serum Institut (SSI) enteric medium for isolation and direct identification of enteric pathogens was evaluated. Sis different biochemical reactions can be read by using the SSI enteric medium, allowing direct identification of a range of enteric pathogens, All 248 gram-negative bacterial species that were tested grew on the SSI enteric medium. Only 10 of 248 bacteria (4%) showed discrepant results in the biochemical reactions, and none of these were enteric pathogens. Forty-three of 47 enteric pathogens (92%) produced identical rates of semiquantitative growth on the SSI enteric medium and 5% blood agar, whereas three Vibrio spp, and one Aeromonas spp, showed reduced growth. Gram-positive bacteria did not grow on the SSI enteric medium, Most enteric pathogens had a detection limit of 50 bacteria per mi of feces, but higher numbers of Vibrio spp, and some Shigella spp, were required for detection. The growth rates of 125 enteric pathogens and 12 Yersinia spp, on the SSI enteric medium, xylose lysine deoxycholate (SLD), Hektoen enteric (HE), Salmonella-Shigella (SS), and cefsulodin-irgasan-novobiocin (CIN) agar were compared, Detection rates after application of 200 CFU were 99% for SSI enteric medium, 92% for SLD, 88% for HE, and 82% for SS agar, The 12 Yersinia spp, grew excellently on both the SSI enteric medium and CIN agar, We conclude that the performance of the SSI enteric medium compares favorably to those of other media tested, Its ability to detect Yersinia spp, may limit the number of media needed in the typical laboratory. The direct identification of enteric pathogens on the medium may also provide a more rapid diagnosis.