Neuroendocrine modulation sustains the C. elegans forward motor state.
Neuroendocrine modulation sustains the C. elegans forward motor state.
复制标题
神经内分泌调节维持线虫前进运动状态
作者：
Lim MA;Chitturi J;Laskova V;Meng J;Findeis D;Wiekenberg A;Mulcahy B;Luo L;Li Y;Lu Y;Hung W;Qu Y;Ho CY;Holmyard D;Ji N;McWhirter R;Samuel AD;Miller DM;Schnabel R;Calarco JA;Zhen M
神经调质塑造神经回路动力学。结合电子显微镜、遗传学、转录组分析、钙成像和光遗传学，我们发现了一个调节C。elegans motor circuit dynamics. Six/SO-家族同源框转录因子β-39控制谱系特异性神经发生以产生神经元RID。RID具有特化内分泌神经元的解剖学特征：它含有几乎唯一的致密核心囊泡，这些囊泡周期性地沿着轴突聚集，并表达多种神经肽，包括FMRF-酰胺相关的FLP-14。RID活动在向前移动时增加。去除RID降低了向前运动的可持续性，这是一种通过去除FLP-14而部分重现的表型。RID的光遗传去极化抑制了向前运动，这种作用在没有FLP-14的情况下减少。总之，这些结果确立了神经内分泌细胞RID在维持C.优雅
Neuromodulators shape neural circuit dynamics. Combining electron microscopy, genetics, transcriptome profiling, calcium imaging, and optogenetics, we discovered a peptidergic neuron that modulates C. elegans motor circuit dynamics. The Six/SO-family homeobox transcription factor UNC-39 governs lineage-specific neurogenesis to give rise to a neuron RID. RID bears the anatomic hallmarks of a specialized endocrine neuron: it harbors near-exclusive dense core vesicles that cluster periodically along the axon, and expresses multiple neuropeptides, including the FMRF-amide-related FLP-14. RID activity increases during forward movement. Ablating RID reduces the sustainability of forward movement, a phenotype partially recapitulated by removing FLP-14. Optogenetic depolarization of RID prolongs forward movement, an effect reduced in the absence of FLP-14. Together, these results establish the role of a neuroendocrine cell RID in sustaining a specific behavioral state in C. elegans.