Cis- and trans-regulation of luteovirus gene expression by the 3' end of the viral genome.
Cis- and trans-regulation of luteovirus gene expression by the 3' end of the viral genome.
复制标题
通过病毒基因组的3'末端对黄体病毒基因表达的顺式调节。
DOI：
10.1016/j.virusres.2015.03.009
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发表时间：
2015-08-03
期刊：
影响因子：
5
通讯作者：
Feng Y
中科院分区：
文献类型：
--
作者：
Miller WA;Jackson J;Feng Y
5.7 kb黄症病毒基因组的翻译受3’非翻译区（UTR）控制。3’ UTR区域与上游数千碱基的序列之间的碱基配对是合成病毒复制酶所需的不依赖帽子结构的翻译和核糖体移码所必需的。黄症病毒产生亚基因组RNA，其可作为mRNA，但一种亚基因组RNA也反式调节翻译起始。与所有病毒一样，3’和5’末端包含被认为有助于RNA合成的结构。本综述描述了大麦黄矮病毒RNA的结构和相互作用，这些结构和相互作用促进了上述事件之间的复杂相互作用，并导致病毒成功感染。我们还展示了一些令人惊讶的结果，即病毒感染细胞或整株植物似乎不需要某些亚基因组RNA。总之，黄症病毒的UTR是高度复杂的实体，其控制并微调病毒复制周期的许多关键事件。
Translation of the 5.7 kb luteovirus genome is controlled by the 3’ untranslated region (UTR). Base pairing between regions of the 3’ UTR and sequences kilobases upstream is required for cap-independent translation and ribosomal frameshifting needed to synthesize the viral replicase. Luteoviruses produce subgenomic RNAs, which can serve as mRNA, but one sgRNA also regulates translation initiation in trans. As on all viruses, the 3’ and 5’ ends contain structures that are presumed to facilitate RNA synthesis. This review describes the structures and interactions of Barley yellow dwarf virus RNA that facilitate the complex interplay between the above events and result in a successful virus infection. We also present surprising results on the apparent lack of need for some subgenomic RNAs for the virus to infect cells or whole plants. In summary, the UTRs of luteoviruses are highly complex entities that control and fine-tune many key events of the virus replication cycle.