Electrochemical Techniques and Applications to Characterize Single‐ and Multicellular Electric Microbial Functions
Electrochemical Techniques and Applications to Characterize Single‐ and Multicellular Electric Microbial Functions
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表征单细胞和多细胞电微生物功能的电化学技术和应用
DOI：
10.1002/9781119611103.ch3
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Okamoto Akihiro
中科院分区：
文献类型：
--
作者：
Saito Junki;Murugan Muralidharan;Deng Xiao;Guionet Alexis;Miran Waheed;Okamoto Akihiro
细胞外电子传递（EET）是微生物在脂质双层膜外的电子传递过程，是由跨膜电子导管介导的。跨学科的兴趣在膜酶的电化学特性，导电纳米线和生物膜，导致了综合方法的发展，研究完整的EET能力的细菌细胞。在本章中，我们总结了技术及其在Shewanella oneidensisMR-1和GeophylsulfurreducensPCA中的应用，这两种方法都是在30年前发现的，并作为模型系统进行了广泛的研究。使用平板电极的简单电化学直接表征细胞表面酶和电极的界面电子传输;一旦与显微技术结合，还可以表征EET相关的代谢过程。交叉指状阵列（IDA）和纳米探针技术将分别引入生物膜和纳米线的电导率表征。还讨论了该领域中存在的一些技术挑战。
The biological electron transport process beyond the insulating lipid bilayer membrane of a microbe, referred to as extracellular electron transport (EET), is mediated by a transmembrane electron conduit. Interdisciplinary interest in the electrochemical properties of the membrane enzyme, and of electrically conductive nanowire and biofilm, led to the development of integrated methodology to study intact EET‐capable bacterial cells. In this chapter, we summarize the techniques and their applications toShewanella oneidensisMR‐1 andGeobacter sulfurreducensPCA, both of which were discovered 30 years ago and have been widely investigated as model systems. Simple electrochemistry with flat electrodes directly characterizes the interfacial electron transport of cell‐surface enzymes and electrodes; and, once combined with microscopic techniques, EET‐associated metabolic processes also can be characterized. Interdigitated array (IDA) and nanoscale probe techniques will be introduced for conductivity characterization of biofilm and nanowire, respectively. Some technical challenges remaining in this field are also addressed.