Effects of cucumber mosaic virus infection on electron transport and antioxidant system in chloroplasts and mitochondria of cucumber and tomato leaves
Effects of cucumber mosaic virus infection on electron transport and antioxidant system in chloroplasts and mitochondria of cucumber and tomato leaves
复制标题
黄瓜花叶病毒感染对黄瓜和番茄叶片叶绿体和线粒体电子传递和抗氧化系统的影响
DOI：
10.1111/j.1399-3054.2008.01189.x
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发表时间：
2009-03-01
影响因子：
6.4
通讯作者：
Yu, Jing-Quan
中科院分区：
文献类型：
--
作者：
Song, Xing-Shun;Wang, Yan-Jie;Yu, Jing-Quan
通过比较黄瓜（Cucumis sativus L.）和番茄（Lycopersicon esculentum Mill.）叶片的气体交换、Chl荧光、呼吸电子传递、超氧化物歧化酶（SOD, EC 1.15.1.1）、抗坏血酸-谷胱甘肽（AsA-GSH）循环酶和H2O2的产生，研究了黄瓜叶片细胞器光合、呼吸电子传递和抗氧化系统对黄瓜花叶病毒（CMV）侵染的响应。病毒侵染和未侵染叶片的线粒体和可溶性组分。CMV长期感染导致光合作用和呼吸速率下降。碳还原的光合电子通量、通过复合体I和复合体II的呼吸电子传递以及细胞呼吸速率均显著降低，而光合替代电子通量和替代呼吸速率均显著增加。这些电子传递的变化伴随着SOD/AsA-GSH循环酶活性的普遍增加，随后是叶绿体和线粒体中H2O2积累的增加。这些结果表明，CMV对光合作用和呼吸电子传递的干扰也会影响抗氧化系统，从而导致各种细胞器的氧化应激。
We examined the responses of the photosynthetic and respiratory electron transport and antioxidant systems in cell organelles of cucumber (Cucumis sativus L.) and tomato (Lycopersicon esculentum Mill.) leaves to infection of cucumber mosaic virus (CMV) by comparing the gas exchange, Chl fluorescence, respiratory electron transport, superoxide dismutase (SOD, EC 1.15.1.1) and ascorbate-glutathione (AsA-GSH) cycle enzymes and the production of H2O2 in chloroplasts, mitochondria and soluble fraction in virus-infected and non-infected leaves. Long-term CMV infection resulted in decreased photosynthesis and respiration rates. Photosynthetic electron flux to carbon reduction, respiratory electron transport via both complex I and complex II and also the Cyt respiration rate all significantly decreased, while photosynthetic alternative electron flux and alternative respiration significantly increased. These changes in electron transport were accompanied by a general increase in the activities of SOD/AsA-GSH cycle enzymes followed by an increased H2O2 accumulation in chloroplasts and mitochondria. These results demonstrated that disturbance of photosynthetic and respiratory electron transport by CMV also affected the antioxidative systems, thereby leading to oxidative stress in various organelles.