REPLICATION OF NONCONJUGATIVE PLASMID RSF1010 IN ESCHERICHIA-COLI K-12
REPLICATION OF NONCONJUGATIVE PLASMID RSF1010 IN ESCHERICHIA-COLI K-12
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DOI：
10.1128/jb.134.3.1117-1122.1978
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发表时间：
1978-01-01
影响因子：
3.2
通讯作者：
FALKOW, S
中科院分区：
文献类型：
--
作者：
DEGRAAFF, J;CROSA, JH;FALKOW, S
用EcoRI限制性内切酶切割非接合F质粒RSF1010（Smr[链霉素抗性]Sur[磺酰胺抗性]）的复制DNA分子并通过EM进行检查。该分析的结果表明，复制是从位于总基因组大小约19%的EcoRI末端的起点开始的。复制以相同的频率单向或双向进行。还提供了含有 Apr[氨苄青霉素抗性]转座序列 (Tn) 的 RSF1010。
Replicating DNA molecules of the nonconjugative F plasmid RSF1010 (Smr [streptomycin resistance] Sur [sulfonamide resistance] were cleaved with the EcoRI restriction endonuclease and examined by EM. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from 1 of the EcoRI ends. Replication proceeded unidirectionally or bidirectionally with equal frequency. Results of the analysis of replicative intermediates of RSF1010 containing the Apr[ampicillin resistance]-transposable sequence (Tn) are also presented.