Rapid and sensitive determination of tryptophan, serotonin and psychoactive tryptamines by thin-layer chromatography/fluorescence detection.
Rapid and sensitive determination of tryptophan, serotonin and psychoactive tryptamines by thin-layer chromatography/fluorescence detection.
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DOI：
10.1016/j.chroma.2007.01.061
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发表时间：
2007-03
期刊：
影响因子：
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通讯作者：
N. Kato;T. Kojima;S. Yoshiyagawa;H. Ohta;A. Toriba;Hideo Nishimura;K. Hayakawa
中科院分区：
文献类型：
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作者：
N. Kato;T. Kojima;S. Yoshiyagawa;H. Ohta;A. Toriba;Hideo Nishimura;K. Hayakawa
建立了一种快速、灵敏、选择性好的薄层荧光检测色氨酸(Trp)、5-羟色胺(5-HT)和精神活性色胺(PATS)的方法。这些化合物在显影底片上用次氯酸钠、过氧化氢或六氰基铁酸钾(III)-氢氧化钠试剂喷洒后加热，形成荧光团。用紫外光(365 Nm)照射，观察到荧光斑点(亮蓝色)。色氨酸、5-羟色胺和PATS的检出限为0.0 1~0.0 6μg，可有效地用于滥用者尿样中没收的PAT粉和PAT的检测。
A rapid, sensitive and selective method for the determination of tryptophan (Trp), serotonin (5-HT) and psychoactive tryptamines (PATs) by thin-layer chromatography (TLC) with fluorescence detection is proposed. These compounds form fluorophores on the developing plate by heating after spraying with sodium hypochlorite, hydrogen peroxide or potassium hexacyanoferrate(III)-sodium hydroxide reagent. Fluorescent spots (vivid blue) were observed by irradiation with ultraviolet light (365nm). The detection limits of Trp, 5-HT and PATs were in the range from 0.01μg to 0.06μg. This method was effectively applied to the detection of confiscated PAT powder and PAT in abusers’ urine samples.