INHIBITION OF CANDIDA-ALBICANS BY THE PEROXIDASE/SCN-/H2O2 SYSTEM
INHIBITION OF CANDIDA-ALBICANS BY THE PEROXIDASE/SCN-/H2O2 SYSTEM
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DOI：
10.1111/j.1399-302x.1992.tb00595.x
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发表时间：
1992-10-01
影响因子：
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通讯作者：
LENANDERLUMIKARI, M
中科院分区：
文献类型：
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作者：
LENANDERLUMIKARI, M
唾液过氧化物酶（SPO）系统对口腔细菌生长、葡萄糖摄取和代谢活动的影响已有文献记载，但对口腔真菌的影响几乎未知。因此，在无菌唾液、磷酸盐缓冲盐水（PBS）和氯化钾中研究了暴露于过氧化物酶/SCN-/H2O2体系下的白色念珠菌（ATCC 28366）的生存能力。pH为5.5时，白色念珠菌的生长速度比pH为7时快。将完整的SPO（或乳酸过氧化物酶）系统添加到灭菌的唾液、KCl （50 muM）或pH为5.5的PBS中，KCl对白色念珠菌的活性有剂量依赖性的抑制作用，但PBS或唾液中没有发现抑制作用。过氧化物酶产生的HOSCN/OSCN-在2小时内达到最大抑制作用。然而，生理唾液浓度的磷酸盐（大于或等于1.0 mM）和PBS阻断了HOSCN/OSCN-的抗真菌作用。SCN-和H2O2的相对比例对抗真菌效果至关重要。当KSCN为0.2 mM时，尽管HOSCN/OSCN-浓度不超过100 muM，但存活能力完全丧失。结论：白色念珠菌对HOSCN/OSCN-敏感，但唾液中磷酸盐的浓度会抑制过氧化物酶系统的抗真菌作用。
Effects of the salivary peroxidase (SPO) system on the growth, glucose uptake and metabolic activities of oral bacteria are well documented but the effects on oral fungi are virtually unknown. Therefore, the viability of Candida albicans (ATCC 28366) exposed to the peroxidase/SCN-/H2O2 system was studied in sterilized saliva, in phosphate-buffered saline (PBS) and in potassium chloride. The growth of C albicans in glucose-supplemented saliva was faster at pH 5.5 than at pH 7. The addition of the complete SPO (or lactoperoxidase) system to either sterilized saliva, KCl (50 muM) or PBS at pH 5.5 inhibited dose-dependently the viability of C. albicans in KCl, but no inhibition was found in PBS or saliva. Maximal inhibition was achieved in 2 h and with > 320 muM of peroxidase-generated HOSCN/OSCN-. However, physiological salivary concentrations of phosphate (greater-than-or-equal-to 1.0 mM) and PBS blocked the antifungal effect of HOSCN/OSCN-. The relative proportions of SCN- and H2O2 were critical to the antifungal effects. With 0.2 mM KSCN, a complete loss of viability was achieved, though the HOSCN/OSCN- concentrations did not exceed 100 muM. It is concluded that C. albicans is sensitive to HOSCN/OSCN- but salivary concentrations of phosphate block the antifungal effect of the peroxidase systems.