The cell-surface marker MTS24 identifies a novel population of follicular keratinocytes with characteristics of progenitor cells
The cell-surface marker MTS24 identifies a novel population of follicular keratinocytes with characteristics of progenitor cells
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DOI：
10.1242/dev.02443
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发表时间：
2006-08-01
期刊：
影响因子：
4.6
通讯作者：
van Ewijk, Willem
中科院分区：
文献类型：
--
作者：
Nijhof, Joanne G. W.;Braun, Kristin M.;van Ewijk, Willem
我们描述了一种新的小鼠祖细胞群体定位于皮脂腺和毛囊隆起之间的一个以前未表征的区域，由其对胸腺上皮祖细胞标志物MTS 24的反应性定义。MTS 24标记毛囊发育早期和成年小鼠中存在的膜结合抗原。MTS 24与α 6-整联蛋白和角蛋白14的表达共定位，表明这些细胞包括基底角质形成细胞。这种新的群体不表达隆起特异性干细胞标志物CD 34或角蛋白15，并且很少保留BrdU标记。通过流式细胞术分离MTS 24阳性和阴性角质形成细胞群体，并评估集落形成效率。与MTS 24阴性基底角质形成细胞相比，MTS 24阳性角质形成细胞的集落形成和集落大小增加了两倍。此外，MTS 24阳性和CD 34阳性亚群在单个细胞克隆连续传代后都能够产生次级集落。最后，比较了MTS 24和CD 34亚群的基因表达谱。这些结果表明，整体基因表达亲。1 e的MTS 24阳性细胞类似于先前在隆突干细胞中报道的模式。总之，这些数据表明，细胞表面标记MTS 24确定了一个新的水库的毛囊角质形成细胞的增殖能力和基因表达促进。也就是说祖细胞或干细胞
We describe a novel murine progenitor cell population localised to a previously uncharacterised region between sebaceous glands and the hair follicle bulge, defined by its reactivity to the thymic epithelial progenitor cell marker MTS24. MTS24 labels a membrane-bound antigen present during the early stages of hair follicle development and in adult mice. MTS24 co-localises with expression of alpha 6-integrin and keratin 14, indicating that these cells include basal keratinocytes. This novel population does not express the bulge-specific stem cell markers CD34 or keratin 15, and is infrequently BrdU label retaining. MTS24-positive and -negative keratinocyte populations were isolated by flow cytometry and assessed for colony-forming efficiency. MTS24-positive keratinocytes exhibited a two-fold increase in colony formation and colony size compared to MTS24-negative basal keratinocytes. In addition, both the MTS24-positive and CD34-positive subpopulations were capable of producing secondary colonies after serial passage of individual cell clones. Finally, gene expression profiles of MTS24 and CD34 subpopulations were compared. These results showed that the overall gene expression pro. le of MTS24-positive cells resembles the pattern previously reported in bulge stem cells. Taken together, these data suggest that the cell-surface marker MTS24 identifies a new reservoir of hair follicle keratinocytes with a proliferative capacity and gene expression pro. le suggestive of progenitor or stem cells.