Generation and Characterization of a Library of Novel Biologically Active Functional Surfactants (Surfmers) Using Combined High-Throughput Methods
Generation and Characterization of a Library of Novel Biologically Active Functional Surfactants (Surfmers) Using Combined High-Throughput Methods
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DOI：
10.1021/acsami.1c08662
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发表时间：
2021-08-31
影响因子：
9.5
通讯作者：
Irvine, Derek J.
中科院分区：
文献类型：
--
作者：
Crucitti, Valentina Cuzzucoli;Contreas, Leonardo;Irvine, Derek J.
我们报告了三种不同的高通量技术的第一次成功组合，以提供加速设计，合成和性能筛选的一个新的，生物指导性的，聚合物，梳状接枝表面活性剂的库。这些三维的表面活性材料被成功地用于通过微流体处理在颗粒表面上形成单分子深层来控制颗粒的表面性质。该策略有意地利用表面活性剂来产生稳定的颗粒并提供所需的细胞指导行为。因此，这些专门设计的高功能表面活性剂对于促进所需的细胞反应至关重要。该文库含有由20种分子上不同的（甲基）丙烯酸单体构建的表面活性剂，其已经通过HT筛选预先鉴定为表现出特异性、变化的和期望的细菌生物膜抑制反应。表面活性剂在水中的自组装性能进行了评估，通过开发一种新的，全自动的，HT方法来确定临界聚集浓度。这些值被用作输入数据，以计算为基础的评价的关键分子描述符，决定聚集行为。因此，HT技术的这种组合通过应用具有复杂分子结构的设计表面活性剂促进了进一步新颖的、高功能的、细胞指导性表面的快速设计、生成和评价。
We report the first successful combination of three distinct high-throughput techniques to deliver the accelerated design, synthesis, and property screening of a library of novel, bio-instructive, polymeric, comb-graft surfactants. These three-dimensional, surface-active materials were successfully used to control the surface properties of particles by forming a unimolecular deep layer on the surface of the particles via microfluidic processing. This strategy deliberately utilizes the surfactant to both create the stable particles and deliver a desired cell-instructive behavior. Therefore, these specifically designed, highly functional surfactants are critical to promoting a desired cell response. This library contained surfactants constructed from 20 molecularly distinct (meth)acrylic monomers, which had been pre-identified by HT screening to exhibit specific, varied, and desirable bacterial biofilm inhibitory responses. The surfactant's self-assembly properties in water were assessed by developing a novel, fully automated, HT method to determine the critical aggregation concentration. These values were used as the input data to a computational-based evaluation of the key molecular descriptors that dictated aggregation behavior. Thus, this combination of HT techniques facilitated the rapid design, generation, and evaluation of further novel, highly functional, cell-instructive surfaces by application of designed surfactants possessing complex molecular architectures.