Resolution enhancement in a light-sheet-based microscope (SPIM)
Resolution enhancement in a light-sheet-based microscope (SPIM)
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DOI：
10.1364/ol.31.001477
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发表时间：
2006-05-15
期刊：
影响因子：
3.6
通讯作者：
Stelzer, Ernst H. K.
中科院分区：
文献类型：
--
作者：
Engelbrecht, Christoph J.;Stelzer, Ernst H. K.
基于光片的显微镜[单平面照明显微镜（SPIM）]在低数值孔径下表现非常好。它补充了目前在现代生命科学中使用的常规（FM），共焦（CFM）和双光子荧光显微镜（2 hv-FM）。横向和轴向SPIM点扩散函数（PSF）的范围进行测量，通过使用荧光珠，以确定3D分辨率。结果进行了比较，来自分析理论和数值模拟的值。发现差异小于5%。SPIM-PSF（10 X /0.3 W）的轴向范围约为5.7 μ m。该值几乎比CFM小2倍，比FM小2.5倍以上，比2 hv-FM小3倍以上。SPIM优于2 ht/-FM和FM，而CFM在NA高于0.8时具有更好的轴向分辨率。(c)2006年美国光学学会。
Light-sheet-based microscopy [single-plane illumination microscope (SPIM)] performs very well at low numerical apertures. It complements conventional (FM), confocal (CFM), and two-photon fluorescence microscopy (2hv-FM) currently used in modern life sciences. Lateral and axial SPIM point spread function (PSF) extents are measured by using fluorescent beads to determine the 3D resolution. The results are compared with values derived from an analytical theory and numerical simulations. The discrepancies are found to be less than 5%. The axial extent of a SPIM-PSF (10 X /0.3 W) is approximately 5.7 mu m. This value is almost a factor of 2 smaller than in CFM, more than 2.5 times smaller than in FM, and more than three times smaller than in 2hv-FM. SPIM outperforms 2ht/-FM and FM, while CFM has a better axial resolution at NAs above 0.8. (c) 2006 Optical Society of America.