Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells
Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells
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DOI：
10.1016/j.biomaterials.2008.04.006
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发表时间：
2008-07-01
期刊：
影响因子：
14
通讯作者：
Kobatake, Eiry
中科院分区：
文献类型：
--
作者：
Nakamura, Makiko;Mie, Masayasu;Kobatake, Eiry
我们开发了人工细胞外基质蛋白，其设计具有胶原结合活性和促进血管内皮细胞网络形成的活性功能单元。我们设计了层粘连蛋白衍生的IKVAV序列，其刺激血管内皮细胞的毛细血管网络形成，以并入弹性蛋白衍生的结构单元。所设计的融合蛋白还具有细胞粘附性RGD序列和来自纤连蛋白的胶原结合结构域。该融合蛋白能与I型胶原结合，促进胶原凝胶的血管生成活性。胶原结合域也有轻微的血管生成活性，然而，设计的融合蛋白也增强细胞迁移活性。设计多功能ECM蛋白的工程策略具有支持当前组织工程技术的可能性。(C)2008爱思唯尔有限公司版权所有。
We developed artificial extracellular matrix proteins designed to have collagen-binding activity and active functional units that promote network formation of vascular endothelial cells. We engineered a laminin-derived IKVAV sequence, which stimulates capillary network formation of vascular endothelial cells, to incorporate into an elastin-derived structural unit. The designed fusion protein also had a cell-adhesive RGD sequence and a collagen-binding domain derived from fibronectin. The resultant fusion protein could bind to collagen type I and promote angiogenic activity of collagen gel. The collagen-binding domain also had slight angiogenic activity; however, the designed fusion protein also enhanced cellular migration activity. The engineering strategy of designing multi-functional ECM proteins has a possibility for supporting current tissue engineering techniques. (C) 2008 Elsevier Ltd. All rights reserved.