Comparison of 4 serological tests--complement fixation, neutralization, fluorescent antibody to membrane antigen and immune adherence hemagglutination--for assay of antibody to varicella-zoster (V-Z) virus.
Comparison of 4 serological tests--complement fixation, neutralization, fluorescent antibody to membrane antigen and immune adherence hemagglutination--for assay of antibody to varicella-zoster (V-Z) virus.
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比较 4 种血清学测试（补体固定、中和、膜抗原荧光抗体和免疫粘附血凝），用于检测水痘带状疱疹 (V-Z) 病毒抗体。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
H. Yabuuchi
中科院分区：
文献类型：
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作者：
A. Yamada;S. Ogino;Y. Asano;T. Otsuka;M. Takahashi;K. Baba;H. Yabuuchi
比较了补体结合试验(CF)、中和试验(NT)、膜抗原荧光抗体试验(FAMA)和免疫黏附血凝试验(IAHA)4种水痘-带状疱疹病毒抗体试验。对52份水痘和带状疱疹患者及水痘活疫苗接种者的血清进行了4项检测。CF试验的敏感性最低，而NT、FAMA和IAHA试验的抗体效价大致相当。IAHA检测是最简单、最快速的方法，适合于V-Z病毒的常规血清学检测。用某儿童机构两次水痘暴发期间的血清，对所有未接种疫苗的儿童均已出现水痘症状的人群进行了IAHA抗体滴度与个体对水痘易感性的回顾性调查。接受IAHA检测的25份未接种疫苗的儿童暴露前血清中，大部分滴度低于1：2。相比之下，23份未出现水痘的接种儿童血清中，所有可检测到的抗体效价为1：2至1：。这些结果表明，IAHA滴度反映了个体对临床水痘的易感性或抵抗力。
Four tests for antibody to varicella-zoster (V-Z) virus were compared; these were tests of complement fixation (CF), neutralization (NT), fluorescent antibody to membrane antigen (FAMA) and immune adherence hemagglutination (IAHA). Fifty-two sera from patients with varicella and zoster and from recipients of live varicella vaccine were examined by the 4 tests. The CF test was least sensitive, but the antibody titers by the NT, FAMA and IAHA tests were roughly comparable. The IAHA test was the simplest and fastest to perform, and appeared suitable for routine serological assay to V-Z virus. The correlation between the IAHA antibody titer and susceptibility of individuals to clinical varicella was investigated retrospectively using sera obtained during 2 outbreaks of varicella in an institution for children, where all the unvaccinated children had developed varicella symptoms. Most of the 25 pre-exposure sera from unvaccinated children examined by the IAHA test had tiers of less than 1:2. In contrast, all the 23 sera from vaccinated children who did not develop varicella had detectable antibody titers of 1:2 to 1:64. These results indicate that the IAHA titer reflects the susceptibility or resistance of individuals to clinical varicella.