Hormone Orchestrates a Hierarchical Transcriptional Cascade That Regulates Al-Induced De Novo Root Regeneration in Tea Nodal Cutting
Hormone Orchestrates a Hierarchical Transcriptional Cascade That Regulates Al-Induced De Novo Root Regeneration in Tea Nodal Cutting
复制标题
激素协调分层转录级联，调节茶节切割中铝诱导的从头根再生
DOI：
10.1021/acs.jafc.1c01100
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发表时间：
2021-05-21
影响因子：
6.1
通讯作者：
Yu, Youben
中科院分区：
文献类型：
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作者：
Cheng, Long;Liu, Huan;Yu, Youben
酸性土壤中的铝对大多数植物都有很强的根毒作用，但对茶树根系的生长和发育却是必不可少的。然而，铝介导的茶树根再生信号通路的分子基础在很大程度上是不清楚的。在这项研究中，我们分析了生理表型，转录组，和植物激素的过程中使用的茎处理铝（0.3 mM）和控制（0.02 mM）。解剖学分析表明，0.3 mM Al处理的茎在7 d内开始发育不定根（AR）原基，21 d后出现AR，而对照表现出明显的延迟。进一步发现，在第3天铝胁迫下，参与ZT、ACC和JA生物合成的多个基因的表达模式均受到铝的刺激;同时，在整个生根过程中，生长素转运蛋白相关基因的表达模式均显著增加。此外，这些基因的表达与ZT，ACC，JA和IAA的积累密切相关。CsERFs，CsMYBs和CsWRKYs转录因子基因在调节AR再生中可能具有关键作用也被发现。我们的研究结果表明，多种植物激素和基因的生物合成形成一个层次的转录级联过程中铝诱导的从头根再生茶树节插。
The aluminum in acid soils is very rhizotoxic to most plant species, but it is essential for root growth and development in Camellia sinensis. However, the molecular basis of Al-mediated signaling pathways in root regeneration of tea plants is largely unclear. In this study, we profiled the physiological phenotype, transcriptome, and phytohormones in the process using stems treated with Al (0.3 mM) and control (0.02 mM). The anatomical analysis showed that the 0.3 mM Al-treated stem began to develop adventitious root (AR) primordia within 7 days, ARs occurred after 21 days, while the control showed a significant delay. We further found that the expression patterns of many genes involved in the biosynthesis of ZT, ACC, and JA were stimulated by Al on day 3; also, the expression profiles of auxin transporter-related genes were markedly increased under Al during the whole rooting process. Moreover, the expression of these genes was strongly correlated with the accumulation of ZT, ACC, JA, and IAA. CsERFs, CsMYBs, and CsWRKYs transcription factor genes with possible crucial roles in regulating AR regeneration were also uncovered. Our findings suggest that multiple phytohormones and genes related to their biosynthesis form a hierarchical transcriptional cascade during Al-induced de novo root regeneration in tea nodal cuttings.